Fabrication of 3D chitosan-hydroxyapatite scaffolds using a robotic dispensing system
Fabrication of 3D chitosan-hydroxyapatite scaffolds using a robotic dispensing system
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DOI：
10.1016/s0928-4931(02)00010-3
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发表时间：
2002-05-31
期刊：
影响因子：
--
通讯作者：
Teoh, SH
中科院分区：
文献类型：
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作者：
Ang, TH;Sultana, FSA;Teoh, SH
设计了一种新型机器人桌面快速成型（RP）系统，用于组织工程支架的制造。实验装置由一个计算机引导的台式机器人和一个单组件气动分配器组成。将点胶材料（壳聚糖和壳聚糖-羟基磷灰石（HA）溶解在乙酸中）储存在一个30毫升的桶中，通过一个小的特氟龙内衬喷嘴将其挤出点胶介质（氢氧化钠-乙醇，比例为7:3）。一层一层地，壳聚糖是用预先编程的布局模式制造的。中和后的壳聚糖形成凝胶状沉淀物，氢氧化钠（NaOH）溶液中的静水压力使长方体支架保持形状。冻干支架与湿支架的线性收缩和体积收缩分别约为31%和62%。层与层之间良好的附着使壳聚糖基质能够形成完全互连的通道结构。体外细胞培养研究结果显示了支架的生物相容性。这项初步研究的结果表明，快速成型机器人点胶（rbod）系统在制造具有规则和可复制大孔结构的三维（31）支架方面具有潜力。(C) 2002 Elsevier Science b.v.版权所有
A new robotic desktop rapid prototyping (RP) system was designed to fabricate scaffolds for tissue engineering applications. The experimental setup consists of a computer-guided desktop robot and a one-component pneumatic dispenser. The dispensing material (chitosan and chitosan-hydroxyapatite (HA) dissolved in acetic acid) was stored in a 30-ml barrel and forced out through a small Teflon-lined nozzle into a dispensing medium (sodium hydroxide-ethanol in ratio of 7:3). Layer-by-layer, the chitosan was fabricated with a preprogramed lay-down pattern. Neutralization of the chitosan forms a gel-like precipitate, and the hydrostatic pressure in the sodium hydroxide (NaOH) solution keeps the cuboid scaffold in shape. Comparison of the freeze-dried scaffold to the wet one showed linear and volumetric shrinkage of about 31% and 62%, respectively. A good attachment between layers allowed the chitosan matrix to form a fully interconnected channel architecture. Results of in vitro cell culture studies revealed the scaffold biocompatibility. The results of this preliminary study using the rapid prototyping robotic dispensing (RPBOD) system demonstrated its potential in fabricating three-dimensional (31)) scaffolds with regular and reproducible macropore architecture. (C) 2002 Elsevier Science B.V All rights reserved.