Arabidopsis ADF1 is Regulated by MYB73 and is Involved in Response to Salt Stress Affecting Actin Filament Organization
Arabidopsis ADF1 is Regulated by MYB73 and is Involved in Response to Salt Stress Affecting Actin Filament Organization
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DOI：
10.1093/pcp/pcab081
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发表时间：
2021-06-04
影响因子：
4.9
通讯作者：
Wang, Xianling
中科院分区：
文献类型：
--
作者：
Wang, Lu;Qiu, Tianqi;Wang, Xianling
肌动蛋白细胞骨架和转录因子在植物对盐胁迫的响应中起着关键作用，然而，对这两种调节因子在盐胁迫响应中的联系知之甚少。肌动蛋白解聚因子（ADF）是真核生物中保守的肌动蛋白结合蛋白。在这里，我们揭示了ADF 1的表达水平受到盐胁迫的诱导。在相同条件下，adf1突变体的存活率显著降低，肌动蛋白索的百分比增加，肌动蛋白丝的密度降低，而ADF1过表达的幼苗则表现出与野生型相反的结果。此外，生化分析显示，MYB 73，R2R3 MYB转录因子，结合到ADF 1的启动子，并通过MYB结合位点核心基序ACCTAC抑制其表达。综上所述，我们的研究结果表明，ADF1通过调节肌动蛋白组织参与盐胁迫，也可能作为一个潜在的下游目标MYB73，这是一个负调节盐胁迫。
Actin cytoskeleton and transcription factors play key roles in plant response to salt stress; however, little is known about the link between the two regulators in response to salt stress. Actin-depolymerizing factors (ADFs) are conserved actin-binding proteins in eukaryotes. Here, we revealed that the expression level of ADF1 was induced by salt stress. The adf1 mutants showed significantly reduced survival rate, increased percentage of actin cable and reduced density of actin filaments, while ADF1 overexpression seedlings displayed the opposite results when compared with WT under the same condition. Furthermore, biochemical assays revealed that MYB73, a R2R3 MYB transcription factor, binds to the promoter of ADF1 and represses its expression via the MYB-binding site core motif ACCTAC. Taken together, our results indicate that ADF1 participates in salt stress by regulating actin organization and may also serve as a potential downstream target of MYB73, which is a negative regulator of salt stress.