Comparative Dynamic Distribution of Avian Infectious Bronchitis Virus M41, H120, and SAIBK Strains by Quantitative Real-Time RT-PCR in SPF Chickens
Comparative Dynamic Distribution of Avian Infectious Bronchitis Virus M41, H120, and SAIBK Strains by Quantitative Real-Time RT-PCR in SPF Chickens
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DOI：
10.1271/bbb.120521
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发表时间：
2012-12-01
影响因子：
1.6
通讯作者：
Yang, Xin
中科院分区：
文献类型：
--
作者：
Fan, Wen-qiao;Wang, Hong-ning;Yang, Xin
禽传染性支气管炎是鸡的一种急性、高度传染性疾病。为研究肾型传染性支气管炎病毒(IBV)SAIBK株与其他毒株(M41株和H120株)动态分布的差异，通过管家基因选择，建立了相对定量实时逆转录聚合酶链式反应(RT-PCR)。本实验选择甘油醛-3-磷酸脱氢酶和泛素进行归一化。对鸡的气管、胸腺、肝、脾、肺、肾、胰腺、腺胃、法氏囊等9种组织进行分析比较，以确定IBV感染的趋向性。在本研究中，建立了肾和肺对IBV感染最敏感的器官。胰腺和肝脏是抗原检测的候选器官。气管和脾可作为组织学诊断参考，但不宜用于抗原检测；腺胃可能是IBV感染的重要靶点；法氏囊和胸腺不是IBV感染的敏感器官。
Avian infectious bronchitis is an acute, highly contagious disease of chickens. To study the differences of dynamic distribution between nephropathogenic infectious bronchitis virus (IBV) strains such as SAIBK and other strains (the M41 and H120 strains), relative quantitative real-time reverse transcription-polymerase chain reaction was developed by housekeeping gene selection. Glyceraldehyde-3-phosphate dehydrogenase and Ubiquitin were chosen for normalization in this experimental set. Then nine tissues, the trachea, thymus, liver, spleen, lungs, kidney, pancreas, proventriculus, and bursa of Fabricius, were analyzed and compared to determine the tropism of IBV infection. In this research, the kidney and the lung were established of the most sensitive organs in IBV infection. The pancreas and the liver are candidates for antigen detection. The trachea and the spleen can be used as references for histological diagnosis, but they are not suitable for antigen detection; proventriculus might be an important target in IBV infection; the thymus and the bursa of Fabricius were not sensitive organs in IBV infection.