Polymorphic microsatellite loci developed from the Hong Kong oyster (Crassostrea hongkongensis).
Polymorphic microsatellite loci developed from the Hong Kong oyster (Crassostrea hongkongensis).
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DOI：
10.4238/gmr.15048676
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发表时间：
2016-10
期刊：
影响因子：
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通讯作者：
Hongshi Ma;Shu Xiao;Y. Zhang;X. M. Li;Junlin Li;Zhisheng Yu
中科院分区：
文献类型：
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作者：
Hongshi Ma;Shu Xiao;Y. Zhang;X. M. Li;Junlin Li;Zhisheng Yu
利用磁珠富集的香港近江牡蛎部分基因组文库，筛选出40个具有多态性的微卫星位点。这些基因座的多态性进行了评估，在30个人从野生种群。多态性标记的等位基因数为2 ~ 13个，平均每个位点5.8个。多态性信息含量范围为0.032 ~ 0.891，其中37个基因座表现出中等或高度多态性。观察到的和预期的杂合度值范围分别为0.033至1.000和0.033至0.931。在40个位点中，有28个位点符合Hardy-Weinberg平衡（HWE），而其余12个位点则显著偏离HWE。这些标记的可用性将有助于未来的遗传研究在C。香港花
Forty polymorphic microsatellite loci were developed from Crassostrea hongkongensis using an enriched partial genomic library with magnetic beads. The polymorphism of these loci was assessed in 30 individuals from a wild population. The allele number of the polymorphic markers ranged from 2 to 13, with an average of 5.8 per locus. The polymorphism information content ranged from 0.032 to 0.891 and 37 loci presented a medium or high level of polymorphism. The observed and expected heterozygosity values ranged from 0.033 to 1.000 and 0.033 to 0.931, respectively. Of the 40 loci, 28 were found to conform to Hardy-Weinberg equilibrium (HWE), whereas the remaining 12 showed a significant departure from HWE. The availability of these markers will aid future genetic studies in C. hongkongensis.