Characterization of bacterial population of raw milk from bovine mastitis by culture-independent PCR-DGGE method
Characterization of bacterial population of raw milk from bovine mastitis by culture-independent PCR-DGGE method
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DOI：
10.1016/j.bej.2009.02.010
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发表时间：
2009-06-01
影响因子：
3.9
通讯作者：
Tanji, Yasunori
中科院分区：
文献类型：
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作者：
Kuang, Ying;Tani, Kaori;Tanji, Yasunori
首次采用非培养PCR-DGGE指纹技术对日本北海道的乳腺炎感染奶牛原料乳中的细菌组成和多样性进行了研究。所有的乳房炎牛奶样品被诊断为单独感染大肠菌群，使用经典的微生物方法后，农场培养。在我们的研究结果中观察到的每个样品之间的群落结构的变化表明，牛乳腺炎相关细菌是宿主特异性的。发现假单胞菌、乳酸乳球菌、金黄色葡萄球菌和埃希氏菌属的成员广泛分布。此外，在一些牛奶样品中发现了一种以上的已知引起乳腺炎的病原体。这些病原体不仅是潜在的病原体，而且还可能在破坏乳腺炎奶牛的自然微生物生态中发挥作用。这一发现突出了传统的鉴定和表征策略的局限性，PCR-DGGE被证明是描述乳房炎乳中细菌植物群，特别是病原体的有力工具。(c)2009爱思唯尔有限公司版权所有。
Culture-independent PCR-DGGE fingerprinting was used to reveal the bacterial composition and diversity associated with raw milk of mastitis-infected cows from Hokkaido,Japan for the first time. All the mastitic milk samples were diagnosed as solely infected by coliforms using the classical microbiological method following on-farm culturing. The variation in community structure between each sample observed in our results indicated that the bovine mastitis-associated bacteria were host-specific. Klebsiella pseudomoniae, Lactococcus lactis, Staphylococcus aureus and members of the Escherichia genus were found to be widely distributed. Furthermore, more than one pathogen known to cause mastitis was found to be present in some milk samples. These pathogens are not only potential etiological agents but may also play a role in disrupting the natural microbial ecology in mastitic cows. This finding highlights the limitation of the traditional identification and characterization strategy, and the PCR-DGGE are shown to be a powerful tool for describing the bacterial flora and especially etiological agents in mastitic milk. (c) 2009 Elsevier B.V. All rights reserved.