Altering the Bubble Release of Reactive Inkjet Printed Silk Micro-rockets
Altering the Bubble Release of Reactive Inkjet Printed Silk Micro-rockets
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DOI：
10.2352/issn.2169-4451.2017.32.452
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发表时间：
2016-09
期刊：
影响因子：
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通讯作者：
D. Gregory;Y. Zhang;Patrick J. Smith;S. Ebbens;Xiubo Zhao
中科院分区：
文献类型：
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作者：
D. Gregory;Y. Zhang;Patrick J. Smith;S. Ebbens;Xiubo Zhao
使用再生丝素蛋白（RSF）的逐层（LBL）反应性喷墨打印（RIJ）的新方法用于产生微米级丝火箭，其具有固定在丝支架结构内的酶过氧化氢酶，并使用过氧化氢酶驱动它们在含有H2O2作为燃料的样品中的运动。通过使用LBL打印方法，我们证明了可以生成3D结构，其中可以将不同的材料在定义的位置并入结构中。丝与喷墨打印方法一起使用具有很大的潜力，可以轻松地将不同的酶，蛋白质，化学物质或其他生物分子结合在一起，并通过将它们捕获到丝支架中来构建多功能设备。这使我们能够产生小规模的设备，可以通过流体环境中的催化反应产生推力，用于潜在的应用，包括环境监测和修复，体内药物输送和修复以及芯片实验室诊断。相比之下，目前的微电机制造工艺通常使用缓慢而冗长的生产工艺（例如蒸发），并结合昂贵的材料，如铂。催化剂在这些装置上的位置已被证明影响轨迹行为，这是不容易控制使用传统的方法。此外，使用铂作为催化剂的装置可经历生物结垢，从而抑制其催化反应。通过使用RIJ创造的生物相容性丝支架，这里产生的设备有可能克服所有这些问题。
A novel approach of using layer-by-layer (LBL) reactive inkjet printing (RIJ) of regenerated silk fibroin (RSF) was used to generate micron-sized silk rockets which have the enzyme catalase immobilised inside the silk scaffold structure and use the catalase enzyme to drive their motion in samples containing H2O2 as a fuel. By using the LBL printing approach we show that is it possible to generate 3D structures where different materials can be incorporated into the structure at defined locations. The use of silk together with an inkjet printing method has great potential to easily incorporate different enzymes, proteins, chemicals or other biomolecules and build versatile devices by entrapping them into the silk scaffold. This allows us to generate small-scale devices that can generate thrust via catalytic reactions within fluidic environments for potential applications including environmental monitoring and remediation, in vivo drug delivery and repair, and lab-on-a-chip diagnostics. In contrast, current manufacturing processes of micromotors often use slow and lengthy production processes (e.g. evaporation) combined with expensive materials such as platinum. The location of catalyst on these devices has been shown to influence trajectory behaviour, which is not easy to control using conventional methods. Furthermore devices using platinum as a catalyst can undergo biofouling thus inhibiting their catalytic reactions. By using biocompatible silk scaffolds, created by RIJ, the devices generated here have the potential to overcome all these problems.