Pigment epithelium-derived factor plays an inhibitory role in proliferation and migration of HaCaT cells
Pigment epithelium-derived factor plays an inhibitory role in proliferation and migration of HaCaT cells
复制标题
色素上皮衍生因子对HaCaT细胞增殖和迁移具有抑制作用
DOI：
10.1007/s11033-010-0336-3
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发表时间：
2011-03-01
影响因子：
2.8
通讯作者：
Zheng, Min
中科院分区：
文献类型：
--
作者：
Li, Chun-Ming;Li, Wei;Zheng, Min
正常的血管系统是通过血管生成因子和抗血管生成因子之间的平衡来维持的。近年来的研究表明，色素上皮衍生因子（PEDF）具有诱导肿瘤分化和抑制肿瘤血管生成的作用。本研究旨在研究PEDF在人角质形成细胞系HaCaT细胞中的表达及其在增殖、粘附和迁移中的作用。我们的研究结果表明，PEDF在HaCaT细胞中表达的mRNA和蛋白质水平分别由RT-PCR和Western blot测定。免疫荧光检测显示PEDF信号主要定位于HaCaT细胞的胞浆内。此外，PEDF的表达被50 ng/ml的VEGF 165降低。PEDF降低HaCaT细胞的增殖和迁移，而HaCaT细胞的粘附上调约29%。PEDF还可诱导HaCaT细胞S期细胞的聚集。此外，PEDF降低了ERK 1/2的磷酸化，而不是JNK和p38。这些结果表明，PEDF可能通过ERK 1/2的去磷酸化对HaCaT细胞的生长和迁移发挥抑制作用。
The normal vasculature is maintained by a balance between angiogenic factors and anti-angiogenic factors. Recent studies have shown that pigment epithelium-derived factor (PEDF) can induce differentiation and inhibit angiogenesis of tumors. This study was designed to investigate the expression of PEDF and its roles in proliferation, adhesion and migration of HaCaT cells, a human keratinocyte cell line. Our results have shown that PEDF is expressed in HaCaT cells at both mRNA and protein levels determined by RT-PCR and Western blot, separately. PEDF signal mainly localizes in the cytoplasm of HaCaT cell, as determined by immunofluorescence. Furthermore, expression of PEDF is decreased by 50 ng/ml of VEGF165. Proliferation and migration of HaCaT cells are decreased by PEDF, while adhesion of HaCaT cells is upregulated approximately by 29%. PEDF also induce the S phase accumulation of HaCaT cells. In addition, phosphorylation of ERK1/2, not JNK and p38, is decreased by PEDF. These results indicate that PEDF may play an inhibitory role on growth and migration of HaCaT cells through dephosphorylation of ERK1/2.