Extracellular Soluble Membranes from Retinal Pigment Epithelial Cells Mediate Apoptosis in Macrophages.
Extracellular Soluble Membranes from Retinal Pigment Epithelial Cells Mediate Apoptosis in Macrophages.
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DOI：
10.3390/cells10051193
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发表时间：
2021-05-13
期刊：
影响因子：
6
通讯作者：
Taylor AW
中科院分区：
文献类型：
--
作者：
Sanjiv N;Osathanugrah P;Fraser E;Ng TF;Taylor AW
视网膜免疫生物学的中心特征是防止巨噬细胞的促炎活性。视网膜色素上皮细胞（RPE）是可溶性抗炎因子的主要来源。这包括当免疫调节神经肽α-黑素细胞刺激激素（α-MSH）的活性被中和时诱导巨噬细胞凋亡的可溶性因子。在这份手稿中，从原发性RPE分离的细胞外可溶性膜（ESM）进行了测定，看看它们是否可以是细胞凋亡的可溶性介质。我们的研究结果表明，RPE ESMs介导的巨噬细胞凋亡的诱导被α-MSH抑制。相比之下，在诱导与原代RPE相似的抗炎活性的条件下培养的RPE系ARPE-19没有激活巨噬细胞中的细胞凋亡。此外，只有来自原代RPE培养物的ESM，而不是来自ARPE-19细胞培养物的ESM，表达mFasL。结果表明，RPE ESMs是一种可溶性凋亡介质，这可能是RPE选择α-MSH诱导的抑制细胞存活的机制。
A central characterization of retinal immunobiology is the prevention of proinflammatory activity by macrophages. The retinal pigment epithelial cells (RPEs) are a major source of soluble anti-inflammatory factors. This includes a soluble factor that induces macrophage apoptosis when the activity of the immunomodulating neuropeptide alpha-melanocyte-stimulating hormone (α-MSH) is neutralized. In this manuscript, isolated extracellular soluble membranes (ESMs) from primary RPE were assayed to see if they could be the soluble mediator of apoptosis. Our results demonstrated that RPE ESMs mediated the induction of macrophage apoptosis that was suppressed by α-MSH. In contrast, the RPE line ARPE-19, cultured under conditions that induce similar anti-inflammatory activity to primary RPEs, did not activate apoptosis in the macrophages. Moreover, only the ESMs from primary RPE cultures, and not those from the ARPE-19 cell cultures, expressed mFasL. The results demonstrate that RPE ESMs are a soluble mediator of apoptosis and that this may be a mechanism by which the RPEs select for the survival of α-MSH-induced suppressor cells.
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影响因子：
5.5
作者：
Taylor AW;Ng TF
通讯作者：
Ng TF
影响因子：
4.6
作者：
Patel, Girijesh Kumar;Khan, Mohammad Aslam;Singh, Ajay Pratap
通讯作者：
Singh, Ajay Pratap
影响因子：
3.7
作者：
Taylor AW
通讯作者：
Taylor AW
DOI：
10.1159/000099265
发表时间：
2007-01-01
期刊：
Chemical immunology and allergy
影响因子：
--
作者：
Ferguson, Thomas A;Griffith, Thomas S
通讯作者：
Griffith, Thomas S
影响因子：
3.4
作者：
Locke, Christina J.;Congrove, Nicole R.;McKay, Brian S.
通讯作者：
McKay, Brian S.