Transcription-dependent spreading of the Dal80 yeast GATA factor across the body of highly expressed genes
Transcription-dependent spreading of the Dal80 yeast GATA factor across the body of highly expressed genes
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DOI：
10.1371/journal.pgen.1007999
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发表时间：
2019-02
期刊：
影响因子：
4.5
通讯作者：
Aria Ronsmans;M. Wéry;Ugo Szachnowski;Camille Gautier;Marc Descrimes;E. Dubois;Antonin Morillon;I. Georis
中科院分区：
文献类型：
--
作者：
Aria Ronsmans;M. Wéry;Ugo Szachnowski;Camille Gautier;Marc Descrimes;E. Dubois;Antonin Morillon;I. Georis
加塔转录因子在真核生物中高度保守，并在涉及癌症进展和造血的基因转录中发挥作用。然而，尽管它们的共有结合位点已经在体外得到了很好的定义，但加塔因子识别的体内选择性仍然很差。使用ChIP-Seq，我们鉴定了酵母中的Dal 80加塔因子靶标。我们的数据揭示Dal 80与大量启动子结合，有时独立于加塔位点，与氮和/或Dal 80敏感性基因表达相关。引人注目的是，Dal 80也在启动子结合的基因体中被检测到，与高表达相关。单基因机制实验表明，Dal 80跨基因体传播需要主动转录。因此，Dal 80与RNA聚合酶II的起始和后起始形式共免疫沉淀。我们的工作表明，加塔因子可以发挥双重，在转录起始和启动后的步骤中发挥协同作用，促进基因表达程序的有效重塑，以应对环境的变化。
GATA transcription factors are highly conserved among eukaryotes and play roles in transcription of genes implicated in cancer progression and hematopoiesis. However, although their consensus binding sites have been well defined in vitro, the in vivo selectivity for recognition by GATA factors remains poorly characterized. Using ChIP-Seq, we identified the Dal80 GATA factor targets in yeast. Our data reveal Dal80 binding to a large set of promoters, sometimes independently of GATA sites, correlating with nitrogen- and/or Dal80-sensitive gene expression. Strikingly, Dal80 was also detected across the body of promoter-bound genes, correlating with high expression. Mechanistic single-gene experiments showed that Dal80 spreading across gene bodies requires active transcription. Consistently, Dal80 co-immunoprecipitated with the initiating and post-initiation forms of RNA Polymerase II. Our work suggests that GATA factors could play dual, synergistic roles during transcription initiation and post-initiation steps, promoting efficient remodeling of the gene expression program in response to environmental changes.