Narrowband Organic Light‐Emitting Diodes for Fluorescence Microscopy and Calcium Imaging
Narrowband Organic Light‐Emitting Diodes for Fluorescence Microscopy and Calcium Imaging
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DOI：
10.1002/adma.201903599
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发表时间：
2019-09
影响因子：
29.4
通讯作者：
Caroline Murawski;A. Mischok;Jonathan H. Booth;J. D. Kumar;Emily Archer;L. Tropf;C. Keum;Yali Deng;Kou Yoshida;I. Samuel;M. Schubert;S. Pulver;M. Gather
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文献类型：
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作者：
Caroline Murawski;A. Mischok;Jonathan H. Booth;J. D. Kumar;Emily Archer;L. Tropf;C. Keum;Yali Deng;Kou Yoshida;I. Samuel;M. Schubert;S. Pulver;M. Gather
荧光成像是生物学中不可或缺的工具，其应用范围从单细胞研究到整个动物研究，以及神经元活动的实时成像目前受到特别关注。为了能够在自由活动的动物中进行细胞尺度的荧光成像，人们开发了可以以微创方式植入的微型显微镜和无透镜成像器；但所涉及的光源的刚性、大小和潜在毒性仍然是一个挑战。在这里，窄带有机发光二极管(OLED)被开发出来，用于活细胞的荧光成像，并通过遗传编码的钙离子指示剂来定位黑腹果蝇的神经元活动。为了避免光谱与样品的荧光重叠，分布式布拉格反射器被集成到OLED上，以阻挡其长波长发射尾巴，这使得图像对比度可与传统的、体积大得多的汞光源相媲美。由于OLED可以在机械柔性衬底上制造并结构成细胞大小的像素阵列，这项工作为开发能够在自由运动的动物中进行功能成像和传感的植入式光源开辟了一条新的途径。
Fluorescence imaging is an indispensable tool in biology, with applications ranging from single‐cell to whole‐animal studies and with live mapping of neuronal activity currently receiving particular attention. To enable fluorescence imaging at cellular scale in freely moving animals, miniaturized microscopes and lensless imagers are developed that can be implanted in a minimally invasive fashion; but the rigidity, size, and potential toxicity of the involved light sources remain a challenge. Here, narrowband organic light‐emitting diodes (OLEDs) are developed and used for fluorescence imaging of live cells and for mapping of neuronal activity in Drosophila melanogaster via genetically encoded Ca2+ indicators. In order to avoid spectral overlap with fluorescence from the sample, distributed Bragg reflectors are integrated onto the OLEDs to block their long‐wavelength emission tail, which enables an image contrast comparable to conventional, much bulkier mercury light sources. As OLEDs can be fabricated on mechanically flexible substrates and structured into arrays of cell‐sized pixels, this work opens a new pathway for the development of implantable light sources that enable functional imaging and sensing in freely moving animals.