Expression of MMIF, HIF-1α and VEGF in Serum and Endometrial Tissues of Patients with Endometriosis
Expression of MMIF, HIF-1α and VEGF in Serum and Endometrial Tissues of Patients with Endometriosis
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DOI：
10.1007/s11596-018-1906-1
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发表时间：
2018-06-01
影响因子：
2.4
通讯作者：
Liu, Li-ping
中科院分区：
文献类型：
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作者：
Zhang, Fan;Liu, Xiao-ling;Liu, Li-ping
本研究旨在探讨巨噬细胞移动抑制因子(MMIF)、缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)在子宫内膜异位症(EM)患者血清和子宫内膜组织中的表达及其临床意义。选择EM患者80例[美国生殖协会I期20例，II期22例，III期21例，IV期17例]，分为轻度痛经组(10~14分)、中度痛经组(16~24分)和重度痛经组(26~30分)。对照组包括40名健康育龄妇女，她们在入选期间接受了常规健康检查。采用双抗体夹心法和免疫印迹法分别检测血清和子宫内膜组织中MMIF、HIF-1α和VEGF的表达。同时检测血清MMIF、HIF-1α、VEGF单独或联合检测的敏感性和特异性。结果表明，EM患者血清MMIF、HIF-1α、VEGF水平显著高于对照组(P<0.05)。三种蛋白在血清和子宫内膜组织中的表达均随着R-AFS分期(P<；0.05)和痛经严重程度(P<；0.05)的增加而显著增加。联合检测血清MMIF、HIF-1α和VEGF的敏感性和特异性均显著高于单项指标检测(P<；0.05)。结论：血清和子宫内膜组织中MMIF、HIF-1α和VEGF的表达可作为判断EM分期和痛经严重程度的指标。联合检测MMIF、HIF-1α和血管内皮生长因子可显著提高诊断的敏感性和特异性。
The aim of this study was to investigate the expression of macrophage migration inhibitory factor (MMIF), hypoxia-inducible factor-1 a (HIF-1 alpha) and vascular endothelial growth factor (VEGF) in the serum and endometrial tissues of patients with endometriosis (EM) and the clinical significance. Eighty EM patients [American Reproductive Association stage I (n=20), stage II (n=22), stage III (n=21) and stage IV (n=17)] were enrolled and divided into mild (10-14 points, n=28), moderate (16-24 points, n=27) and severe (26-30 points, n=25) dysmenorrhea groups. The control group included 40 healthy women of childbearing age who underwent routine healthcare examinations in the enrolment period. The expression of MMIF, HIF-1 alpha and VEGF in the serum and endometrial tissues was measured by enzyme-linked immunosorbent assay and Western blotting, respectively. Meanwhile, the sensitivity and specificity of serum MMIF, HIF-1 alpha, and VEGF when separately used as single indexes or jointly used as one index were examined as well. The results showed that serum concentrations of MMIF, HIF-1 alpha, and VEGF were significantly higher in EM patients than in controls (P < 0.05). The expression of all three proteins in both serum and endometrial tissues increased significantly with the R-AFS stage (P < 0.05) and with dysmenorrheal severity (P < 0.05). The sensitivity and specificity of the combined detection of serum MMIF, HIF-1 alpha, and VEGF levels were significantly higher than those of single index detection (P < 0.05). In conclusion, the expression of MMIF, HIF-1 alpha, and VEGF in the serum and endometrial tissues may be used to assess the stage of EM and the severity of dysmenorrhea. Combined evaluation of MMIF, HIF-1 alpha, and VEGF significantly improves the diagnostic sensitivity and specificity.