THE DISKS-LARGE TUMOR SUPPRESSOR GENE OF DROSOPHILA ENCODES A GUANYLATE KINASE HOMOLOG LOCALIZED AT SEPTATE JUNCTIONS
THE DISKS-LARGE TUMOR SUPPRESSOR GENE OF DROSOPHILA ENCODES A GUANYLATE KINASE HOMOLOG LOCALIZED AT SEPTATE JUNCTIONS
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DOI：
10.1016/0092-8674(81)90009-x
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发表时间：
1991-08-09
期刊：
影响因子：
64.5
通讯作者：
BRYANT, PJ
中科院分区：
文献类型：
--
作者：
WOODS, DF;BRYANT, PJ
果蝇致死性(1)discs large-1 (dlg)肿瘤抑制基因的突变导致成虫盘肿瘤性过度生长。近全长 cDNA 的测序预测蛋白质含有与酵母鸟苷酸激酶同源的结构域和与 SH3 同源的区域，SH3 是非受体蛋白酪氨酸激酶和其他信号转导蛋白中的假定调节基序。使用针对融合肽的抗体进行的免疫荧光分析表明，dlg 基因产物位于侧细胞膜的顶带中，位于隔膜连接处的位置。结果表明，涉及鸟嘌呤核苷酸的信号转导过程发生在隔膜连接处，并且对于果蝇上皮细胞增殖控制是必需的。
Mutations of the lethal(1)discs large-1 (dlg) tumor suppressor gene of Drosophila cause neoplastic overgrowth of the imaginal discs. Sequencing of a near full-length cDNA predicts a protein containing a domain homologous to yeast guanylate kinase and a region homologous to SH3, a putative regulatory motif in nonreceptor protein tyrosine kinases and other signal transduction proteins. Immunofluorescence analysis using antibodies directed against fusion peptides shows that the dlg gene product is localized in an apical belt of the lateral cell membrane, at the position of the septate junction. The results suggest that a signal transduction process involving guanine nucleotides occurs at the septate junction and is necessary for cell proliferation control in Drosophila epithelia.