Specificity of retrograde transport of nerve growth factor (NGF) in sensory neurons: A biochemical and morphological study
Specificity of retrograde transport of nerve growth factor (NGF) in sensory neurons: A biochemical and morphological study
复制标题
感觉神经元中神经生长因子（NGF）逆行转运的特异性：生化和形态学研究
DOI：
10.1016/0006-8993(75)90129-8
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发表时间：
1975
期刊：
影响因子：
2.9
通讯作者：
H. Thoenen
中科院分区：
文献类型：
--
作者：
K. Stoeckel;M. Schwab;H. Thoenen
以前的研究表明，神经生长因子(NGF)被肾上腺素能神经末梢高选择性地摄取，并被逆行运输到核周11、22。本实验的目的是研究感觉神经元在整个生命周期中是否表现出同样的高选择性逆行运输，尽管已知它们对NGF的剧烈反应仅限于个体发育的短时期。然而，这种优先积累在注射后6h内不能检测到，并达到最大值(注射侧与非注射侧的比率，5：1)11-16小时后，切断臂丛神经，在注射[~(125)I]NGF之前局部注射秋水仙碱，极大地减少了放射性在注射侧神经节的优先聚集。经计算，神经生长因子在感觉神经元的逆行转运速度为13 mm/h，约为肾上腺素能神经元的5倍。通过注射~(125)I标记的牛血清白蛋白和细胞色素c并未导致放射性在注射侧感觉神经节的优先聚集，证明了这种逆行运输的选择性前爪注射[~(125)I]NGF后，注射侧感觉神经节(C6~C7)内的细胞。只有属于大细胞类型的细胞才被标记。从光镜下的形态计量学研究可以看出，在10日龄大鼠的前爪内长期注射神经生长因子(7μg/g/d，超过5天)并没有引起感觉神经元的亢进反应。
In previous studies it has been shown that nerve growth factor (NGF) is taken up with a high selectivity by adrenergic nerve terminals and is transported retrogradely to the perikaryon11,22.It was the aim of the present experiments to investigate whether the sensory neurons exhibit the same high degree of selectivity for retrograde transport throughout the whole life cycle, although it is known that their dramatic response to NGF is confined to a short period of ontogenetic development.Unilateral injection of [125I]NGF into the forepaw of adult rats was followed by a preferential accumulation of radioactivity in the sensory ganglia (C6–C7) of the injected side. However, this preferential accumulation was not detectable earlier than 6 h after injection and reached a maximum (ratio between injected and non-injected side, 5:1) after 11–16 h. Transection of the plexus brachialis abolished and local administration of colchicine prior to that of [125I]NGF greatly reduced the preferential accumulation of radioactivity in the ganglia of the injected side.The rate of retrograde transport of NGF in sensory neurons was calculated to be 13 mm/h which is about 5 times faster than that in adrenergic neurons.The selectivity of this retrograde transport was demonstrated by the fact that injection of125I-labeled bovine serum albumin and cytochrome c did not result in a preferential accumulation of radioactivity in the sensory ganglia of the injected side.Light microscopic autoradiography revealed heavily labeled cells in the sensory ganglia (C6–C7) of the injected side after administration of [125I]NGF into the forepaw. Only cells belonging to the large cell type were labeled. Prolonged (7 μg/g/day over 5 days) injection of NGF into the forepaw of 10-day-old rats did not result in a hypertropic response of the sensory neurons as far as can be judged from morphometric studies at the light microscopic level.