Vaginal self-swab specimen collection in a home-based survey of older women: methods and applications.
Vaginal self-swab specimen collection in a home-based survey of older women: methods and applications.
复制标题
老年妇女家庭调查中的阴道自拭标本采集：方法和应用。
DOI：
10.1093/geronb/gbn021
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Jordan,JeanneA
中科院分区：
文献类型：
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作者：
Lindau,StacyT;Hoffmann,JoscelynN;Lundeen,Katie;Jaszczak,Angela;McClintock,MarthaK;Jordan,JeanneA
ObjectivesTo描述所使用的方法，合作，进行化验，和应用的阴道标本收集老年妇女在他们的homes. MethodsCommunity居住的妇女（N= 1,550），年龄57-85岁，参加了一个全国性的代表性概率调查。2005年至2006年期间进行了阴道自我拭子标本采集和家庭访谈。分析标本的细菌性阴道病（BV）、阴道念珠菌病（VC）、高危型人乳头瘤病毒（HR-HPV）和细胞学特征。现场的方法，同意程序，拭子协议，实验室程序，和结果reporting.ResultsOne千二十八名受访者（67.5%加权）同意提供阴道标本，99.1%是成功的。标本充足率为BV和VC，94.1%; HR-HPV，99.7%;细胞学，85.5%。最常见的记录原因是身体或健康问题（38%的无应答者）。应答者比无应答者更有可能更年轻，受教育程度更高，更有可能报告最近的盆腔检查，绝经期激素使用和最近的性活动。DiscussionCollection阴道自拭子标本从老年妇女在人口为基础的研究是可行的，并提供了新的数据有关的女性生殖道的微环境特征的分析妇科健康，性活动和问题，以及免疫和炎症功能。
ObjectivesTo describe the methods used for, cooperation with, assays conducted on, and applications of vaginal specimens collected by older women in their homes.MethodsCommunity-residing women (N= 1,550), ages 57–85 years, participated in a nationally representative probability survey. Vaginal self-swab specimen collection and in-home interviews were conducted between 2005 and 2006. Specimens were analyzed for bacterial vaginosis (BV), vaginal candidiasis (VC), high-risk human papillomavirus (HR-HPV), and cytological characteristics. Field methods, consent procedures, the swab protocol, laboratory procedures, and results reporting are described.ResultsOne thousand twenty-eight respondents (67.5% weighted) agreed to provide a vaginal specimen; 99.1% were successful. The specimen adequacy rates were BV and VC, 94.1%; HR-HPV, 99.7%; and cytology, 85.5%. The most common recorded reason for nonparticipation was a physical or health problem (38% of nonresponders). Responders were significantly more likely than nonresponders to be younger and more educated, and were more likely to report a recent pelvic examination, menopausal hormone use, and recent sexual activity.DiscussionCollection of vaginal self-swab specimens from older women in a population-based study is feasible and provides novel data on microenvironmental characteristics of the female genital tract relevant to analyses of gynecologic health, sexual activity and problems, and immune and inflammatory function.