Capsular polysaccharides and lipopolysaccharides from two Bacteroides fragilis reference strains: chemical and immunochemical characterization
Capsular polysaccharides and lipopolysaccharides from two Bacteroides fragilis reference strains: chemical and immunochemical characterization
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两种脆弱拟杆菌参考菌株的荚膜多糖和脂多糖：化学和免疫化学表征
DOI：
10.1128/jb.153.2.991-997.1983
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发表时间：
1983
影响因子：
3.2
通讯作者：
J. Lönngren
中科院分区：
文献类型：
--
作者：
D. Kasper;A. Weintraub;A. Lindberg;J. Lönngren
脆弱拟杆菌在含有1%葡萄糖和10%胎牛血清的复杂培养基中，在可控条件下发酵罐生长，细菌产量高。经苯酚-水热萃取后，从水相分离出荚膜多糖，用3%脱氧胆酸钠缓冲液用Sephacryl S-300层析纯化。采用苯酚-氯仿-轻石油醚萃取法分离脂多糖。脆弱芽孢杆菌NCTC 9343的荚膜多糖含有6种糖：L-焦糖、D-半乳糖、D-和L-喹诺亚胺、D-氨基葡萄糖和半乳糖醛酸。菌株ATCC 23745的胶囊还含有d -葡萄糖、L- focusamine、L-鼠李糖胺和3-氨基-3,6-二脱氧己糖，但缺乏d -喹诺糖胺。后者也含有丙氨酸（4%）。ELISA法对荚膜多糖进行免疫化学检测。两菌株的脂多糖均含有相同的糖（l-鼠李糖、d -葡萄糖、d -半乳糖和d -氨基葡萄糖）和脂肪酸（13-甲基-十四烷酸、3-羟基-十六烷酸和3-羟基-15甲基-十六烷酸为主要成分），且ELISA抑制结果相同。
Fermentor growth of Bacteroides fragilis under controlled conditions in a complex medium containing 1% glucose and 10% fetal calf serum resulted in high yields of bacteria. After hot phenol-water extraction of the organisms, capsular polysaccharide was isolated from the aqueous phase and purified by Sephacryl S-300 chromatography in a buffer with 3% sodium deoxycholate. Lipopolysaccharide was isolated by phenol-chloroform-light petroleum ether extraction. The capsular polysaccharide from B. fragilis strain NCTC 9343 contained six sugars: L-fucose, D-galactose, D- and L-quinovosamine, D-glucosamine, and galacturonic acid. The capsule of strain ATCC 23745 also contained D-glucose, L-fucosamine, L-rhamnosamine, and a 3-amino-3,6-dideoxyhexose but lacked D-quinovosamine. The latter capsule also contained alanine (4%). The capsular polysaccharides were different immunochemically by ELISA inhibition. The lipopolysaccharide of both strains contained the same sugars (L-rhamnose, D-glucose, D-galactose, and D-glucosamine) and fatty acids (13-methyl-tetradecanoic and 3-hydroxy-hexadecanoic and 3-hydroxy-15 methyl-hexadecanoic as major constituents) and were identical by ELISA inhibition.