42 degrees C heat stress pretreatment protects human melanocytes against 308-nm laser-induced DNA damage in vitro
42 degrees C heat stress pretreatment protects human melanocytes against 308-nm laser-induced DNA damage in vitro
复制标题
42 摄氏度热应激预处理可保护人类黑素细胞免受 308 nm 激光诱导的体外 DNA 损伤
DOI：
10.1007/s10103-020-03012-3
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发表时间：
2020
影响因子：
2.1
通讯作者：
Gu Weijie
中科院分区：
文献类型：
--
作者：
Hu Wenzhi;Mi Na;Xu Yanfang;Zhao Guang;Gu Weijie
白癜风是由于功能性黑素细胞丧失而引起的一种常见的皮肤病。近年来，308 nm氯化氙准分子激光(UVB激光)光疗被广泛应用于白癜风的治疗。然而，过量的UVB会引起黑素细胞的光损伤和光致癌。以前的研究表明，热对UVB诱导的黑素细胞损伤具有保护作用。在本研究中，我们将热应激预处理与UVB结合起来，以评估热应激预处理是否对UVB诱导的损伤有改善作用。培养人原代黑素细胞(HMCs)，用308 nm激光进行照射，并对其进行热处理或不加热。采用四甲基偶氮唑盐比色法、细胞凋亡率分析和彗星试验检测HMCS的损伤情况。免疫印迹和免疫荧光染色检测HSP70的表达和亚细胞定位。在42℃下加热1h的HMCs没有细胞毒性。此外，预热处理减轻了UVB激光诱导的损伤，减少了DNA损伤，减少了细胞凋亡。HSP70的水平和定位决定了其对UVB诱导的DNA损伤的保护作用。联合预热处理和308 nm氯化氙准分子激光是一种潜在的治疗方法，不仅可以促进白癜风的复色，还可以减少UVB诱导的光损伤。
Vitiligo is a common depigment of skin disorder due to loss of functional melanocytes. Recently, the phototherapy with a 308-nm xenon-chloride excimer laser (UVB laser) is wildly used in vitiligo treatment. However, excessive UVB will induce photo-damage and photo-carcinogenesis in melanocytes. Previous studies revealed a protective effect of heat on UVB-induced melanocyte damage. In this study, we combined heat stress pretreatment with UVB to evaluate whether heat stress pretreatment has an ameliorative effect on UVB-induced damage. Human primary melanocytes (HMCs) were cultured and irradiated with a 308-nm laser with/without heat treatment. MTT assay, apoptosis analysis, and comet assay were conducted to monitor the damage of HMCs. Western blot and immunofluorescence staining were performed to assess the expression and subcellular localization of HSP70. HMCs heated at 42 °C for 1 h exhibit no cytotoxicity. Furthermore, preheat treatment attenuated the UVB laser-induced injury, reduced the DNA damage, and attenuated the cell apoptosis. The level and the localization of HSP70 determined the protective effects against UVB-induced DNA damage. Combining preheat treatment with a 308-nm xenon-chloride excimer laser would be a potential therapeutic method not only promotes the repigment of vitiligo but also reduces the UVB-induced photo-damage.