Analysis of mixed biofilm (Staphylococcus aureus and Pseudomonas aeruginosa) by laser ablation electrospray ionization mass spectrometry
Analysis of mixed biofilm (Staphylococcus aureus and Pseudomonas aeruginosa) by laser ablation electrospray ionization mass spectrometry
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DOI：
10.1080/08927014.2015.1011067
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发表时间：
2015-01-01
期刊：
影响因子：
2.7
通讯作者：
van Hoek, Monique L.
中科院分区：
文献类型：
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作者：
Dean, Scott N.;Walsh, Callee;van Hoek, Monique L.
铜绿假单胞菌和金黄色葡萄球菌是普遍存在的病原体，经常在多种微生物的生物膜相关感染中一起发现。这项研究是第一个使用激光消融电喷雾电离质谱（LAESI-MS）快速研究混合生物膜内的细菌。快速，直接，非侵入性LAESI-MS分析生物膜可以显着加快生物膜的研究，并提供以前无法获得的信息，生物膜组成和生物膜处理的影响。LAESI-MS直接应用于多微生物生物膜，并分析铜绿假单胞菌和S。金黄色葡萄球菌在混合生物膜内共定位或自分离。LAESI-MS还用于分析LL-37抗微生物肽处理生物膜后的离子。这种环境电离方法为未来的生物膜研究提供了希望。这种创新技术的使用对生物膜的研究具有深远的意义，因为LAESI-MS消除了对冗长和破坏性样品制备的需要，同时允许对未固定和湿生物膜进行快速分析。
Pseudomonas aeruginosa and Staphylococcus aureus are ubiquitous pathogens often found together in polymicrobial, biofilm-associated infections. This study is the first to use laser ablation electrospray ionization mass spectrometry (LAESI-MS) to rapidly study bacteria within a mixed biofilm. Fast, direct, non-invasive LAESI-MS analysis of biofilm could significantly accelerate biofilm studies and provide previously unavailable information on both biofilm composition and the effects of antibiofilm treatment. LAESI-MS was applied directly to a polymicrobial biofilm and analyzed with respect to whether P. aeruginosa and S. aureus were co-localized or self-segregated within the mixed biofilm. LAESI-MS was also used to analyze ions following LL-37 antimicrobial peptide treatment of the biofilm. This ambient ionization method holds promise for future biofilm studies. The use of this innovative technique has profound implications for the study of biofilms, as LAESI-MS eliminates the need for lengthy and disruptive sample preparation while permitting rapid analysis of unfixed and wet biofilms.