Effects of transient PTH on early proliferation, apoptosis, and subsequent differentiation of osteoblast in primary osteoblast cultures.
Effects of transient PTH on early proliferation, apoptosis, and subsequent differentiation of osteoblast in primary osteoblast cultures.
复制标题
瞬时 PTH 对原代成骨细胞培养物中成骨细胞早期增殖、凋亡和随后分化的影响。
DOI：
10.1152/ajpendo.00216.2006
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Rowe,DavidW
中科院分区：
文献类型：
--
作者：
Wang,Yu-Hsiung;Liu,Yaling;Rowe,DavidW
In primary calvarial osteoblast cultures derived from transgenic mice expressing green fluorescent protein (GFP) under the control of 3.6-kb Col1a1 promoter, the emergence of GFP signal marks the transition of multipotential osteoprogenitors into preosteoblasts. Early transient treatment (days 1–7) of these cultures with parathyroid hormone (PTH) has an anabolic effect that is not associated with an increase in total DNA content or cell number inday 21cultures. In the present study, the effect of early PTH treatment on cell proliferation and apoptosis was examined in greater detail in GFP(+) and GFP(−) cells using flow cytometry. In preconfluent cultures, PTH significantly reduced the proportion of cells in S phase but increased those in G0/G1and G2+M phases in both GFP(+) and GFP(−) subpopulations. PTH decreased apoptosis only in GFP(−) but not GFP(+) cells, indicating an increased survival of GFP(−) cells. In contrast, PTH did not change the amounts of cell proliferation and apoptosis seen in either compartment after these cultures reached confluence. To further assess the effect of early PTH treatment on osteogenic differentiation, secondary cultures of sorted GFP(+) or GFP(−) cells were obtained fromday 7primary cultures that had been treated for 1 wk with PTH. This treatment resulted in larger areas of GFP expression accompanied by increased xylenol orange/von Kossa staining in the secondary cultures of GFP fractions. Early transient PTH treatment appears to enhance the commitment of progenitor cells to an osteogenic fate and results in a higher proportion of cells that achieve full osteoblast differentiation.
