METAPHASE CHROMOSOME STRUCTURE - BANDS ARISE FROM A DIFFERENTIAL FOLDING PATH OF THE HIGHLY AT-RICH SCAFFOLD
METAPHASE CHROMOSOME STRUCTURE - BANDS ARISE FROM A DIFFERENTIAL FOLDING PATH OF THE HIGHLY AT-RICH SCAFFOLD
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DOI：
10.1016/0092-8674(94)90502-9
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发表时间：
1994-02-25
期刊：
影响因子：
64.5
通讯作者：
LAEMMLI, UK
中科院分区：
文献类型：
--
作者：
SAITOH, Y;LAEMMLI, UK
使用高度AT特异性荧光染料道诺霉素，纵向光学信号称为AT队列，被认为是从高度AT-丰富的支架相关区域（SAR）的脚手架的阵容中产生的，被确定在天然染色体。荧光显带被认为是由AT队列在其从端粒到端粒的过程中的差异折叠路径引起的。AT队列在Q带中紧密卷曲或折叠，由此产生的横贯染色单体的横纹，也代表Giemsa子带，在Q区产生明亮的富含AT的信号。相比之下，R带包含更中心的（未折叠的）AT队列，在R区域上产生AT-迟钝信号。AT队列通过针对拓扑异构酶II（topo II）和HMG-I/Y作为天然染色体的支架的免疫荧光来鉴定;来自这两种蛋白质的荧光信号类似于详细的Q型带型。天然染色体似乎是根据环支架模型组装的。
Using the highly AT-specific fluorchrome daunomycin, a longitudinal optical signal called AT queue, thought to arise from a line-up of the highly AT-rich scaffold-associated regions (SARs) by the scaffolding, was identified in native chromosomes. Fluorescence banding is proposed to result from a differential folding path of the AT queue during its progression from telomere to telomere. The AT queue is tightly coiled or folded in a Q band, the resulting transverse striations across the chromatid, which also represent Giemsa subbands, generating a bright AT-rich signal over the Q region. The R bands, in contrast, contain a more central (unfolded) AT queue, yielding an AT-dull signal over the R regions. The AT queue is identified by immunofluorescence against topoisomerase II (topo II) and HMG-I/Y as the scaffold of native chromosomes; the fluorescence signal from both proteins is akin to a detailed Q-type banding pattern. Native chromosomes appear assembled according to the loop-scaffold model.