CHARACTERIZATION AND FUNCTIONAL-STUDIES OF RHEUMATOID SYNOVIAL MAST-CELLS - ACTIVATION BY SECRETAGOGUES, ANTI-IGE, AND A HISTAMINE-RELEASING LYMPHOKINE
CHARACTERIZATION AND FUNCTIONAL-STUDIES OF RHEUMATOID SYNOVIAL MAST-CELLS - ACTIVATION BY SECRETAGOGUES, ANTI-IGE, AND A HISTAMINE-RELEASING LYMPHOKINE
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DOI：
10.1002/art.1780290802
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发表时间：
1986-08-01
影响因子：
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通讯作者：
KAPLAN, AP
中科院分区：
文献类型：
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作者：
GRUBER, B;POZNANSKY, M;KAPLAN, AP
对35例类风湿性关节炎(RA)和7例骨性关节炎滑膜标本的光镜分析显示，肥大细胞在血管周围、纤维间质区域和淋巴集合体周围聚集。异染性染色、免疫荧光研究和超微结构分析显示，单个结缔组织型肥大细胞群具有表面IgE受体。滑膜组织可提取组胺总量为4.15±-。RA滑膜2.30微克/克(n=8)，0.53微克/克(n=8)。骨性关节炎滑膜0.23微克/克(n=7)。用抗IgE抗体(32.3%)、化合物48/80(40.1%)、钙离子载体A23187(25.2%)和部分纯化的具有组胺释放活性的淋巴因子(23.9%)刺激RA滑膜肥大细胞，检测肥大细胞分泌的组胺。
Microscopic analysis of synovial specimens from 35 patients with rheumatoid arthritis (RA) and 7 patients with osteoarthritis revealed mast cell hyperplasia in perivascular regions, in fibrous interstitial areas, and clustered around the periphery of lymphoid aggregates. Metachromatic staining, immunofluorescence studies, and ultrastructural analysis revealed a single population of connective tissue-type mast cells with surface IgE receptors. Total extractable histamine of synovial tissue was 4.15 .+-. 2.30 .mu.g/gm (n = 8) for RA synovium and 0.53 .+-. 0.23 .mu.g/gm (n = 7) for OA synovium. Mast cell secretion was assessed and specific release of histamine from RA synovial mast cells was observed following stimulation with anti-IgE (32.3%), compound 48/80 (40.1%), calcium ionophore A23187 (25.2%), and a partially purified lymphokine with histamine-releasing activity (23.9%).