Estradiol regulates the expression of CD45 splicing isoforms in lymphocytes
Estradiol regulates the expression of CD45 splicing isoforms in lymphocytes
复制标题
雌二醇调节淋巴细胞中 CD45 剪接异构体的表达
DOI：
10.1007/s11033-020-05373-y
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发表时间：
2020-03
影响因子：
2.8
通讯作者：
Zhongjing Su
中科院分区：
文献类型：
--
作者：
Tianwei Zhang;Zhaoxia Dong;Haitao Cai;Ju Rong;Zhongjing Su
CD45, a common leukocyte antigen expressed on the surface of all nucleated hematopoietic cells, indicates the developmental stage and functional status of lymphocytes by its alternative splicing isoforms. Estrogen is correlated with the immune activity of lymphocytes and is involved in the sex bias of several human autoimmune diseases, but the effect of estrogen on the expression of the CD45 splicing isoforms remains unknown. In the present study, a potential estrogen response element was identified on the opposite strand of the CD45 gene by bioinformatics software prediction. The results from RT-qPCR results showed that the expression levels of CD45RO isoform and CD45 antisense RNA were increased after the lymphocytes were treated with 10 nM 17beta-estradiol, and this effect of 17beta-estradiol was reversed when the lymphocytes were cotreated with an estrogen receptor antagonist. Moreover, bisulfite sequencing PCR showed that CD45 DNA methylation in lymphocytes was increased after the treatment with 10 nM 17beta-estradiol. In conclusion, estradiol regulated the expression of CD45 in an estrogen receptor-dependent manner and was associated with CD45 antisense RNA and DNA methylation. The results helped elucidate the regulatory mechanism of the expression of CD45 isoforms and the correlation between estrogen levels and immune activity in females.
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影响因子：
14.9
作者：
Davis-Turak J;Johnson TL;Hoffmann A
通讯作者：
Hoffmann A
影响因子：
30.5
作者：
Chang VT;Fernandes RA;Ganzinger KA;Lee SF;Siebold C;McColl J;Jönsson P;Palayret M;Harlos K;Coles CH;Jones EY;Lui Y;Huang E;Gilbert RJC;Klenerman D;Aricescu AR;Davis SJ
通讯作者：
Davis SJ
DOI：
10.1016/j.bbrc.2013.06.108
发表时间：
2013-08
影响因子：
3.1
作者：
S. Chandrasekharan;Krishnaveni Kandasamy;Pavithra Dayalan;V. Ramamurthy
通讯作者：
S. Chandrasekharan;Krishnaveni Kandasamy;Pavithra Dayalan;V. Ramamurthy
不同雌激素剂量对培养神经干细胞增殖和分化的影响。
影响因子：
1.5
作者：
Zhang Lixia;Ma Yaqun;Liu Min;Ma Yulong;Guo Hang
通讯作者：
Guo Hang
影响因子：
64.8
作者：
Shukla S;Kavak E;Gregory M;Imashimizu M;Shutinoski B;Kashlev M;Oberdoerffer P;Sandberg R;Oberdoerffer S
通讯作者：
Oberdoerffer S