Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus
Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus
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DOI：
10.1016/j.cj.2022.11.001
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发表时间：
2022-12
期刊：
影响因子：
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通讯作者：
Y. Tan;Lanyang Ren;Jia Wang;Shuyao Ran;Liusha Wu;Ziyi Cheng;C. Qu;Jiana Li;Liezhao Liu-Liezhao-Li
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文献类型：
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作者：
Y. Tan;Lanyang Ren;Jia Wang;Shuyao Ran;Liusha Wu;Ziyi Cheng;C. Qu;Jiana Li;Liezhao Liu-Liezhao-Li
叶片是油菜光合作用的主要器官，其形态直接影响油菜的光合效率，为提高油菜种植密度和产量提供了重要的技术支持。然而，对甘蓝型油菜叶片形态的分子调控机制了解甚少，制约了该性状育种的进展。我们描述了一个新的显性突变体，卷曲叶1（cl1），它赋予不均匀的背腹轴发育，不规则的细胞结构和影响向重力反应在苗期。利用SSR标记将CL 1定位在A05染色体上1.573 Mb的区域内，并与卷曲F2群体中的植株表型共分离。在该突变体中，IAA2蛋白的高度保守的降解决定子基序（GWSPV）中发现了一个取代（P62S），该取代削弱了生长素存在下IAA2与TIR 1之间的相互作用，影响了生长素信号传导。P62S置换诱导的卷曲叶表型通过BnaA05.iaa2在拟南芥和B.油菜。我们的研究结果解释了IAA2在油菜中的功能，为进一步研究生长素信号传导和油菜叶片发育的机制奠定了基础。油菜。
The leaf is the main organ for rapeseed photosynthesis, and its morphology influences photosynthetic efficiency and supports increased planting density and yield. However, the molecular regulatory mechanism of leaf morphology inBrassica napusis poorly understood, restricting progress in breeding for the trait. We describe a novel dominant mutation,curly leaf 1(cl1), which confers uneven dorsal–ventral axis development, irregular cellular structure and influenced gravitropic response in the seedling stage. TheCL1locus was mapped to a 1.573-Mb interval on chromosome A05 using simple sequence repeat (SSR) markers, and co-segregated with the phenotype of plants in the curly F2population. A substitution (P62S) was identified in the highly conserved degron motif (GWSPV) of the IAA2 protein in thecl1mutant, and the P62S substitution impaired the interaction between IAA2 and TIR1 in the presence of auxin, influencing auxin signaling. The P62S substitution-induced curly leaf phenotype was verified by ectopic expression ofBnaA05.iaa2inArabidopsisandB. napus. Our findings explain the function ofIAA2in rapeseed, providing a foundation for future investigation of auxin signaling and the mechanisms underlying leaf development inB. napus.