Analysis of histamine-producing cells at the late phase of allergic inflammationin rats
Analysis of histamine-producing cells at the late phase of allergic inflammationin rats
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DOI：
10.1046/j.1365-2567.2000.00986.x
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发表时间：
2000-04-01
期刊：
影响因子：
6.4
通讯作者：
Ohuchi, K
中科院分区：
文献类型：
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作者：
Shiraishi, M;Hirasawa, N;Ohuchi, K
为了鉴定过敏性炎症晚期的组胺产生细胞，在炎症部位的浸润性白细胞中检测了L-组氨酸脱羧酶（HDC）的表达。与未免疫大鼠的浸润性白细胞相比，免疫大鼠（将抗原溶液注射到气囊中）的气囊液中浸润性白细胞中的HDC活性和HDC mRNA水平增加。当培养抗原注射后8小时收集的浸润白细胞时，来自免疫大鼠的细胞的组胺产生高于来自非免疫大鼠的细胞。HDC mRNA的原位杂交结果显示，免疫组大鼠在注射抗原后4小时，几乎所有浸润的白细胞均高强度表达HDC mRNA，而非免疫组大鼠仅显示弱强度的HDC mRNA。在免疫大鼠中，在4小时时袋液中浸润的白细胞中约90%为中性粒细胞，8%为单核细胞/巨噬细胞。在浸润的白细胞中未检测到肥大细胞和嗜碱性粒细胞。当大鼠腹膜中性粒细胞孵育的12-O-十四烷酰佛波醇13-乙酸酯的存在下，组胺的产生显着增加。这些结果表明，白细胞，主要是中性粒细胞，浸润在炎症部位负责组胺的产生在过敏性炎症的晚期阶段。
To identify histamine-producing cells at the late phase of allergic inflammation, the expression of l-histidine decarboxylase (HDC) was examined in the infiltrating leucocytes in the inflammatory locus. HDC activity and HDC mRNA levels in the infiltrating leucocytes in the pouch fluid of the immunized rats (that were injected with the antigen solution into the air pouch) were increased compared with those in the infiltrating leucocytes of the non-immunized rats. When infiltrating leucocytes collected 8 hr after antigen injection were cultured, histamine production by the cells from the immunized rats was higher than that from the non-immunized rats. In situ hybridization of HDC mRNA revealed that almost all the infiltrating leucocytes of the immunized rats, 4 hr after injection of the antigen, expressed HDC mRNA with high intensity, while those of the non-immunized rats showed only a weak intensity of HDC mRNA. In the immunized rats, approximate to 90% of leucocytes infiltrating in the pouch fluid at 4 hr were neutrophils and 8% were monocytes/macrophages. Neither mast cells nor basophils were detected in the infiltrating leucocytes. When rat peritoneal neutrophils were incubated in the presence of 12-O-tetradecanoylphorbol 13-acetate, histamine production was significantly increased. These findings suggest that the leucocytes, mainly neutrophils, infiltrating at the inflammatory locus are responsible for histamine production at the late phase of allergic inflammation.