Pathogenicity of an emergent, ovine abortifacient Campylobacter jejuni clone orally inoculated into pregnant guinea pigs
Pathogenicity of an emergent, ovine abortifacient Campylobacter jejuni clone orally inoculated into pregnant guinea pigs
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DOI：
10.2460/ajvr.70.10.1269
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发表时间：
2009-10-01
影响因子：
1
通讯作者：
Yaeger, Michael J.
中科院分区：
文献类型：
--
作者：
Burrough, Eric R.;Sahin, Orhan;Yaeger, Michael J.
目的：比较一种紧急流产药空肠弯曲杆菌（ia3902）与对照菌株在妊娠豚鼠口服接种后的致病性。动物——58只怀孕的豚鼠。12只动物与5只假接种的对照动物一起接种C空肠IA 3902，以确认流产潜力。一旦确认致病性，通过口服接种进行攻毒，其中12只豚鼠接种了IA 3902, 12只接种了从羊粪便中分离的C空肠（OF48）， 12只接种了完全测序的人类空肠分离物（NCTC 11168）， 5只作为假接种的对照动物。流产后，豚鼠被安乐死；收集样本进行微生物培养、组织学检查和免疫组织化学分析。结果12只动物接种空肠ia3902诱导流产，10只动物口服诱导6只动物流产。口服接种后，3株菌株均在肠道定植，但只有ia3902菌株导致流产。ia3902和NCTC 11168均存在感染证据；然而，空肠C只在接种ia3902的动物的胎胎盘单位中恢复。免疫组织化学分析将空肠C i3902感染定位于胎盘下滋养细胞、血管周围组织和胎盘过渡区吞噬细胞。结论和临床意义：本研究揭示空肠C i3902是一种独特的高流产力菌株，由于其对胎儿胎盘单位的亲和力，能够定植肠道，诱导全身感染并导致流产。该弯曲杆菌可有效地用于豚鼠口服接种败血性流产的研究。（动物医学杂志2009；70:1269-1276）
Objective-To compare pathogenicity of an emergent abortifacient Campylobacter jejuni (IA 3902) with that of reference strains after oral inoculation in pregnant guinea pigs.Animals-58 pregnant guinea pigs.Procedures-12 animals were challenged IP with C jejuni IA 3902 along with 5 sham-inoculated control animals to confirm abortifacient potential. Once pathogenicity was confirmed, challenge via oral inoculation was performed whereby 12 guinea pigs received IA 3902, 12 received C jejuni isolated from ovine feces (OF48), 12 received a fully sequenced human Cjejuni isolate (NCTC 11168), and 5 were sham-inoculated control animals. After abortions, guinea pigs were euthanized; samples were collected for microbial culture, histologic examination, and immunohistochemical analysis.Results-C jejuni IA 3902 induced abortion in all 12 animals following IP inoculation and 6 of 10 animals challenged orally. All 3 isolates colonized the intestines after oral inoculation, but only IA 3902 induced abortion. Evidence of infection existed for both IA 3902 and NCTC 11168; however, C jejuni was only recovered from fetoplacental units of animals inoculated with IA 3902. Immunohistochemical analysis localized C jejuni IA 3902 infection to subplacental trophoblasts, perivascular tissues, and phagocytes in the placental transitional zone.Conclusions and Clinical Relevance-This study revealed that C jejuni IA 3902 was a unique, highly abortifacient strain with the ability to colonize the intestines, induce systemic infection, and cause abortion because of its affinity for the fetoplacental unit. Guinea pigs could be effectively used in the study of septic abortion after oral inoculation with this Campylobacter strain. (Am J Vet Res 2009;70:1269-1276)