Flow cytometric assays for quantifying activated ovine platelets
Flow cytometric assays for quantifying activated ovine platelets
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DOI：
10.1111/j.1525-1594.2007.00498.x
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发表时间：
2008-02-01
影响因子：
2.4
通讯作者：
Wagner, William R.
中科院分区：
文献类型：
--
作者：
Johnson, Carl A., Jr.;Snyder, Trevor A.;Wagner, William R.
绵羊是心血管装置临床前评估的常见动物模型，包括心脏瓣膜、血管内移植物和心室辅助装置。生物相容性对于这些设备的成功至关重要；然而，评估绵羊生物相容性的工具有限。为了满足这一需求，评估了与活化的人和牛血小板以及膜联蛋白 V 蛋白结合的抗体与活化的羊血小板的潜在交叉反应性。这些候选标记物与受刺激和静止的绵羊全血一起孵育，并通过流式细胞术定量与血小板的结合。几种抗人 CD62P 抗体，包括一种多克隆抗体、三种单克隆抗体和膜联蛋白 V，选择性地与活化的绵羊血小板结合。还开发了一种量化血小板微聚集体的测定方法。量化羊血小板活化的检测方法可以提高羊模型人造器官临床前开发过程中获得的生物相容性数据的质量，可能有助于评估设计改进以增强设备的生物相容性。
Ovines are a common animal model for preclinical evaluation of cardiovascular devices including heart valves, endovascular grafts, and ventricular assist devices. Biocompatibility is essential to the success of these devices; however, tools to assess biocompatibility in ovines are limited. To address this need, antibodies that bind to activated human and bovine platelets and annexin V protein were evaluated for potential cross-reactivity to activated ovine platelets. These candidate markers were incubated with stimulated and quiescent ovine whole blood, and binding to platelets was quantified by flow cytometry. Several antihuman CD62P antibodies including one polyclonal antibody, three monoclonal antibodies, and annexin V selectively bound to activated ovine platelets. An assay to quantify platelet microaggregates was also developed. The availability of assays to quantify ovine platelet activation can increase the quality of biocompatibility data obtainable during preclinical development of artificial organs in the ovine model, potentially aiding in the evaluation of design refinements to enhance device biocompatibility.