Genome-wide analysis of transcription factor binding sites and their characteristic DNA structures.
Genome-wide analysis of transcription factor binding sites and their characteristic DNA structures.
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转录因子结合位点及其特征 DNA 结构的全基因组分析
DOI：
10.1186/1471-2164-16-s3-s8
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发表时间：
2015
期刊：
影响因子：
4.4
通讯作者：
Xiong Y
中科院分区：
文献类型：
--
作者：
Dai Z;Guo D;Dai X;Xiong Y
转录因子（TF）通过结合DNA调控区来调节基因表达。转录因子结合位点（TFBS）不仅在一级DNA序列中是保守的，而且在DNA结构中也是保守的。然而，TF和它们的优选DNA结构之间的全局关系仍有待阐明。在本文中，我们已经开发了一种计算方法来产生一个全基因组的景观的TF和它们的特征结合的DNA结构在酿酒酵母。我们揭示了不同TF的DNA结构特征。TFBS的结构保守性表现出位置偏好性。DNA序列的结构水平与TF-DNA结合亲和力相关。我们提供了全基因组范围内的对应的TF的DNA结构。我们的研究结果将有助于理解TF的调控机制。
Transcription factors (TF) regulate gene expression by binding DNA regulatory regions. Transcription factor binding sites (TFBSs) are conserved not only in primary DNA sequences but also in DNA structures. However, the global relationship between TFs and their preferred DNA structures remains to be elucidated. In this paper, we have developed a computational method to generate a genome-wide landscape of TFs and their characteristic binding DNA structures in Saccharomyces cerevisiae. We revealed DNA structural features for different TFs. The structural conservation shows positional preference in TFBSs. Structural levels of DNA sequences are correlated with TF-DNA binding affinities. We provided the genome-wide correspondences of TFs to DNA structures. Our findings will have implications in understanding TF regulatory mechanisms.