[13N] Ammonia Positron Emission Tomographic/Computed Tomographic Imaging Targeting Glutamine Synthetase Expression in Prostate Cancer
[13N] Ammonia Positron Emission Tomographic/Computed Tomographic Imaging Targeting Glutamine Synthetase Expression in Prostate Cancer
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DOI：
10.2310/7290.2014.00048
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发表时间：
2015-03-01
影响因子：
2.8
通讯作者：
He, Qiao
中科院分区：
文献类型：
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作者：
Shi, Xinchong;Zhang, Xiangsong;He, Qiao
本研究的目的是探讨谷氨酰胺合成酶（GS）在前列腺癌（PCa）中的表达，以及[N-13]氨正电子发射断层扫描/计算机断层扫描（PET/CT）在前列腺癌成像中的应用。测定PC3和DU145细胞对[N-13]氨的吸收率和GS的表达。34例疑似PCa患者行[N-13]氨PET/CT成像，并行GS免疫组化染色。PC3和DU145细胞对[N-13]氨的摄取随着培养基中谷氨酰胺的减少而增加。当谷氨酰胺被剥夺时，GS信使核糖核酸和蛋白的表达也增加。活检样本中，前列腺癌组织中GS表达评分明显高于良性组织（p < 0.001）， GS最高表达评分与Gleason评分呈正相关（Spearman r = 0.52）。在34例患者中，PCa节段的[N-13]氨吸收量显著高于良性节段(p
The purpose of this study was to investigate the expression of glutamine synthetase (GS) in prostate cancer (PCa) and the utility of [N-13] ammonia positron emission tomography/computed tomography (PET/CT) in the imaging of PCa. The uptake ratio of [N-13] ammonia and the expression of GS in PC3 and DU145 cells was measured. Thirty-four patients with suspected PCa underwent [N-13] ammonia PET/CT imaging, and immunohistochemistry staining of GS was performed. The uptake of [N-13] ammonia in PC3 and DU145 cells elevated along with the decrease in glutamine in medium. The expression of GS messenger ribonucleic acid and protein also increased when glutamine was deprived. In biopsy samples, the GS expression scores were significantly higher in PCa tissue than in benign tissues (p < .001), and there was a positive correlation between the maximum GS expression scores and Gleason scores (Spearman r = .52). In 34 patients, [N-13] ammonia uptake in PCa segments was significantly higher than that in benign segments (p