Inhibitory mechanisms of Acacia mearnsii extracts on the growth of Microcystis aeruginosa.
Inhibitory mechanisms of Acacia mearnsii extracts on the growth of Microcystis aeruginosa.
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DOI：
10.2166/wst.2015.038
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发表时间：
2015-03
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影响因子：
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通讯作者：
Zhibin Liu;Lirong Zhou;Dandan Liu;Qiyu Zhu;Wenqing Chen
中科院分区：
文献类型：
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作者：
Zhibin Liu;Lirong Zhou;Dandan Liu;Qiyu Zhu;Wenqing Chen
我们之前的工作表明，黑荆提取物可以抑制铜绿微囊藻的生长，铜绿微囊藻是富营养化淡水中形成有毒蓝藻水华的常见物种。在本研究中，我们证明该植物提取物能显著提高细胞膜的通透性和膜上的钙⁺/镁⁺-三磷酸腺苷酶活性。长期暴露在浓度为20ppm的曼氏拟青霉提取液中，会导致藻细胞膜渗漏甚至溶解。比较植物提取物处理前后3个光合作用相关基因(rbcL、psaB和psbD)在铜绿假单胞菌中的表达，发现植物提取物显著降低了它们的表达。光合作用相关基因的下调可能表明光合作用过程受到抑制。因此，我们的结果表明，黑曲霉提取物对铜绿假单胞菌的光合作用系统和细胞膜都有潜在的破坏作用。
Our previous work revealed that Acacia mearnsii extract can inhibit the growth of Microcystis aeruginosa, the common species forming toxic cyanobacterial blooms in eutrophic freshwater. In the present study, we demonstrated that this plant extract can significantly increase cell membrane permeability and Ca²⁺/Mg²⁺-ATPase activity on the membrane. Long-term exposure to concentrations of 20 ppm A. mearnsii extract led to algal cell membrane leakage or even lysis. Comparison of expression of three photosynthesis-related genes (rbcL, psaB and psbD) in M. aeruginosa with and without plant extract treatment revealed that their expression was remarkably reduced in the presence of the extract. Down-regulation of photosynthesis-related genes could indicate the inhibition of the photosynthetic process. Thus, our results suggested that both photosynthetic systems and membranes of M. aeruginosa are potentially damaged by A. mearnsii extract.