The complete genome sequence and analysis of the human pathogen Campylobacter lari
The complete genome sequence and analysis of the human pathogen Campylobacter lari
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DOI：
10.1089/fpd.2008.0101
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发表时间：
2008-08-01
影响因子：
2.8
通讯作者：
Parker, Craig T.
中科院分区：
文献类型：
--
作者：
Miller, William G.;Wang, Guilin;Parker, Craig T.
拉氏弯曲杆菌是变形菌门的一个分支，是耐热弯曲杆菌属的一部分，该分支包括人类病原体C。空肠。在这里，我们展示了人类临床分离株C.的完整基因组序列。拉里RM2100。菌株RM2100的基因组类似于1.53 Mb，并包括46 kb的大质粒pCL 2100。在菌株RM 2100基因组中还存在一个36 kb的推定原噬菌体，称为CLIE 1，其类似于CJIE 4，CJIE 4是一种存在于C.空肠RM1221基因组。几乎所有（90%）的基因内容是菌株RM2100与其他特征性耐热弯曲杆菌基因组中存在的基因相似。然而，先前在其他弯曲杆菌基因组中鉴定的涉及氨基酸生物合成和能量代谢的几个基因在C.拉里RM2100基因组。因此，C. Lari RM2100被预测为多重营养缺陷型，不能合成八种不同氨基酸、乙酰辅酶A和泛酸。此外，菌株RM2100不包含完整的TCA循环，并且缺失呼吸链的CydAB末端氧化酶。这种生物体中氨基酸生物合成途径的缺陷可以通过大量编码的肽酶来潜在地补偿。尽管如此，RM2100菌株中某些关键酶功能的明显缺失预计会对C.拉里生物学也可能是C. Lari的代谢机制与其环境活动范围和寄主选择有关。
Campylobacter lari is a member of the epsilon subdivision of the Proteobacteria and is part of the thermotolerant Campylobacter group, a clade that includes that includes the human pathogen C. jejuni. Here we present the complete genome sequence of the human clinical isolate, C. lari RM2100. The genome of strain RM2100 is similar to 1.53 Mb and includes the 46 kb megaplasmid pCL2100. Also present within the strain RM2100 genome is a 36 kb putative prophage, termed CLIE1, which is similar to CJIE4, a putative prophage present within the C. jejuni RM1221 genome. Nearly all (90%) of the gene content is strain RM2100 is similar to genes present in the genomes of other characterized thermotolerant campylobacters. However, several genes involved in amino acid biosynthesis and energy metabolism, identified previously in other Campylobacter genomes, are absent from the C. lari RM2100 genome. Therefore, C. lari RM2100 is predicted to be multiply auxotrphic, unable to synthesize eight different amino acids, acetyl-coA, and pantothenate. Additionally, strain RM2100 does not contain a complete TCA cycle and is missing CydAB terminal oxidase of the respiratory chain. Defects in the amino acid biosynthetic pathways in this organism could be potentially compensated by the large number of encoded peptidases. Nevertheless, the apparent absence of certain key enzymatic functions in strain RM2100 would be expected to have an impact on C. lari biology. It is also possible that the reduction in the C. lari metabolic machinery is related to its environmental range and host preference.