Comparison between ballooned hepatocytes occurring in human alcoholic and nonalcoholic liver diseases.
Comparison between ballooned hepatocytes occurring in human alcoholic and nonalcoholic liver diseases.
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人类酒精性和非酒精性肝病中发生的气球状肝细胞的比较。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
S. Takase
中科院分区：
文献类型：
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作者：
Y. Matsuda;A. Takada;H. Sato;M. Yasuhara;S. Takase
为明确酒精性肝病与非酒精性肝病肝细胞气球样变的发病机制差异，用形态计量学和生物化学方法对肝微管进行了观察，并对肝切片进行了转铁蛋白染色。等电聚焦免疫固定法检测血清转铁蛋白的微异质性。酒精性肝病患者肝微管明显减少，转铁蛋白在酒精性肝病气球样变的肝细胞中明显染色，而非酒精性肝病患者则无此现象。转铁蛋白染色的程度与肝微管含量有关，也与酒精性肝病血清转铁蛋白微异质性的出现有关。这些研究结果表明，酒精性肝病肝细胞气球样变，而不是在非酒精性肝病，是由于微管聚合受损的可输出蛋白质的积累。这种积累可能与微管功能受损抑制糖基化和糖蛋白分泌有关。根据这些结果，可以确定，肝脏中转铁蛋白的染色可能有助于肝脏疾病病因的鉴别。
To establish clearly what the pathogenetic differences are in the hepatocytic ballooning between human alcoholic and nonalcoholic liver diseases, hepatic microtubules were examined by morphometric and biochemical methods, and staining of transferrin was carried out on liver sections immunohistochemically. Microheterogeneity of serum transferrin was also detected by immunofixation after isoelectric focusing. Hepatic microtubules were significantly decreased in alcoholic liver disease, and transferrin was clearly stained in the ballooned hepatocytes of alcoholic liver disease but not in nonalcoholic liver disease. The degree to which transferrin was stained was related to hepatic microtubular contents and also related to the appearance of the microheterogeneity of serum transferrin in alcoholic liver disease. These findings indicate that ballooning of hepatocytes in alcoholic liver disease, but not in nonalcoholic liver disease, is caused by the accumulation of exportable proteins due to impairment of microtubular polymerization. This accumulation might be related to the inhibition of glycosylation and secretion of glycoproteins by the impairment of microtubular functions. From these results, it was determined that staining of transferrin in the liver may be useful for differentiation of the etiology of liver diseases.