Evaluating soil management with community-level physiological microbial profiles
Evaluating soil management with community-level physiological microbial profiles
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DOI：
10.1016/j.apsoil.2004.09.007
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发表时间：
2005-05-01
影响因子：
4.8
通讯作者：
Lanyon, LE
中科院分区：
文献类型：
--
作者：
Bucher, AE;Lanyon, LE
作物轮作和作物生产投入等土壤管理措施会影响土壤微生物群落。碳底物利用模式已被用来区分不同生态系统中微生物群落的功能活性。通过基于革兰氏阴性Biolog(R)平板反应的微生物群落水平生理图谱(CLPP)分析，评估了长期轮作试验(HRE)和宾夕法尼亚州农场的微生物群落功能活性。孵育72 h后，对底物反应进行主成分分析和聚类分析。在HRE中，施有机肥和轮作作物的处理与不施肥料和连续种植玉米的处理形成了不同的集群。有常规有机修正的农场与没有或很少添加有机添加剂的农场形成了不同的集群。作为土壤管理对微生物群落活动影响的指标，全平板指标--平均平板强度(MPI)、C底物反应次数--尤其有效。增施有机肥和轮作处理的HRE底物利用多样性不同，但不同管理方式的农田基质利用多样性差异不大。虽然并非没有限制，但微生物群落水平的生理图谱可以作为一种工具来探索不同土壤管理对土壤微生物群落功能活动的影响。(C)2004爱思唯尔B.V.保留所有权利。
Soil management practices such as crop rotations and crop production inputs can affect soil microbial communities. Carbon substrate utilization patterns have been used to distinguish microbial community functional activity in different ecosystems. Microbial community functional activity from a long-term crop rotation experiment (HRE) and from Pennsylvania farms was evaluated by analysis of microbial community-level physiological profiles (CLPP) based on Gram-negative Biolog (R) plate reactions. Principal component analysis (PCA) and cluster analyses of substrate reactions were analyzed after 72 h of incubation. In the HRE, treatments with manure and crop rotations formed a different cluster than treatments with no manure and continuous corn. Farms with routine organic amendments formed a different cluster than farms with few or no organic additions. Whole plate metrics-mean plate intensity (MPI), number of C substrate reactions-were particularly effective as indicators of soil management impacts on microbial community activity. Substrate utilization diversity was different in treatments with manure additions and crop rotations treatments of HRE, but not for farms with different management. Although not without limitation, microbial community-level physiological profiling can be used as a tool to explore the impacts of different soil management on the functional activity of soil microbial communities. (c) 2004 Elsevier B.V. All rights reserved.