Genetic analysis of rice mutants responsible for narrow leaf phenotype and reduced vein number
Genetic analysis of rice mutants responsible for narrow leaf phenotype and reduced vein number
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DOI：
10.1266/ggs.16-00018
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发表时间：
2016-08-01
影响因子：
1.1
通讯作者：
Hirano, Hiro-Yuki
中科院分区：
文献类型：
--
作者：
Kubo, Fumika Clara;Yasui, Yukiko;Hirano, Hiro-Yuki
叶是光合作用的主要场所，也是植物构型的关键决定因素。水稻叶薄而细长，由叶片和叶鞘组成，叶鞘由叶片节分开。两种类型的脉管系统，即大维管束和小维管束，与一个坚固的结构，中脉一起平行运行。在本文中，我们研究了四个基因的功能，调节叶片的宽度和脉数：窄叶1（NAL1），NAL2，NAL3和NAL7。我们将这些基因的原始突变体与标准野生型水稻台中65回交。然后，我们比较了每个突变对相似遗传背景的影响，并研究了这些基因的遗传相互作用。甲单突变和nal2 nal3双突变对叶宽表现出严重的影响，导致非常窄的叶。虽然静脉数也减少了指甲和nal2 nal3突变体，小静脉数减少更强烈的比大静脉数。相比之下，nal7突变体对叶片宽度和脉数的影响较小，并且大脉和小脉都受到类似的影响。因此，窄叶表型的基因似乎发挥不同的作用。nal7突变体与nal2 nal3双突变体或nal2 nal3单突变体组合时，对叶片宽度和脉数均表现出加性效应。此外，内部组织的观察结果表明，细胞分化是部分受损的nal2 nal3 nal7突变体，在这个三重突变体的叶宽严重减少一致。
Leaves are a major site for photosynthesis and a key determinant of plant architecture. Rice produces thin and slender leaves, which consist of the leaf blade and leaf sheath separated by the lamina joint. Two types of vasculature, the large and small vascular bundles, run in parallel, together with a strong structure, the midrib. In this paper, we examined the function of four genes that regulate the width of the leaf blade and the vein number: NARROW LEAF1 (NAL1), NAL2, NAL3 and NAL7. We backcrossed original mutants of these genes with the standard wild type rice, Taichung 65. We then compared the effect of each mutation on similar genetic backgrounds and examined genetic interactions of these genes. The nail single mutation and the nal2 nal3 double mutation showed a severe effect on leaf width, resulting in very narrow leaves. Although vein number was also reduced in the nail and nal2 nal3 mutants, the small vein number was more strongly reduced than the large vein number. In contrast, the nal7 mutation showed a milder effect on leaf width and vein number, and both the large and small veins were similarly affected. Thus, the genes responsible for narrow leaf phenotype seem to play distinct roles. The nal7 mutation showed additive effects on both leaf width and vein number, when combined with the nail single or the nal2 nal3 double mutation. In addition, observations of inner tissues revealed that cell differentiation was partially compromised in the nal2 nal3 nal7 mutant, consistent with the severe reduction in leaf width in this triple mutant.