Thylakoid Membrane Reduction Affects the Photosystem Stoichiometry in the Cyanobacterium Synechocystis sp PCC 6803
Thylakoid Membrane Reduction Affects the Photosystem Stoichiometry in the Cyanobacterium Synechocystis sp PCC 6803
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DOI：
10.1104/pp.108.132373
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发表时间：
2009-02-01
期刊：
影响因子：
7.4
通讯作者：
Schneider, Dirk
中科院分区：
文献类型：
--
作者：
Fuhrmann, Eva;Gathmann, Sven;Schneider, Dirk
叶绿体和蓝细菌中类囊体膜的生物发生至今仍不清楚。质体中的囊泡诱导蛋白1（Vipp 1）被认为是拟南芥（Arabidopsis thaliana）以及蓝细菌集胞藻（Synechocystis sp. PCC 6803）中类囊体膜形成所必需的，尽管其确切的生理功能至今仍不清楚。在这里，我们报告说，在集胞藻细胞中的Vipp 1耗尽后，在单个集胞藻细胞中的类囊体层的数量减少，特别是，光系统I（PSI）复合物的内容是高度减少类囊体。此外，天然光合复合物的分离表明，PSI三聚体是不稳定的和单体物种是丰富的。因此，类囊体膜的耗竭特别影响蓝藻中PSI的生物发生和/或稳定。
Biogenesis of thylakoid membranes in both chloroplasts and cyanobacteria is largely not understood today. The vesicle-inducing protein in plastids 1 (Vipp1) has been suggested to be essential for thylakoid membrane formation in Arabidopsis (Arabidopsis thaliana), as well as in the cyanobacterium Synechocystis sp. PCC 6803, although its exact physiological function remains elusive so far. Here, we report that, upon depletion of Vipp1 in Synechocystis cells, the number of thylakoid layers in individual Synechocystis cells decreased, and that, in particular, the content of photosystem I (PSI) complexes was highly diminished in thylakoids. Furthermore, separation of native photosynthetic complexes indicated that PSI trimers are destabilized and the monomeric species is enriched. Therefore, depletion of thylakoid membranes specifically affects biogenesis and/or stabilization of PSI in cyanobacteria.