Color reversion of the albino medaka fish associated with spontaneous somatic excision of the Tol-1 transposable element from the tyrosinase gene.
Color reversion of the albino medaka fish associated with spontaneous somatic excision of the Tol-1 transposable element from the tyrosinase gene.
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DOI：
10.1111/j.1600-0749.2006.00300.x
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发表时间：
2006-06
期刊：
影响因子：
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通讯作者：
M. Tsutsumi;S. Imai;Y. Kyono-Hamaguchi;S. Hamaguchi;A. Koga;H. Hori
中科院分区：
文献类型：
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作者：
M. Tsutsumi;S. Imai;Y. Kyono-Hamaguchi;S. Hamaguchi;A. Koga;H. Hori
青鳉鱼白化突变体 i(1) 是富田收集的青鳉色素沉着突变体之一，由于插入转座元件 Tol-1 导致酪氨酸酶基因失活，因此表现出完整的白化表型。最近，我们实验室的一个养殖种群中出现了马赛克黑色 i(1) 青鳉鱼。在所有组织中都观察到了它们的色素细胞，包括眼睛和皮肤，其中在野生型中可以检测到黑色素。在这项研究中，我们分析了回复体的酪氨酸酶基因，结果显示 Tol-1 已从该基因中被精确切除，这表明存在因果关系。色素沉着的马赛克图案表明该元素从酪氨酸酶基因中自发体细胞切除。据我们所知，这是第一个在脊椎动物表型上表现出具有体细胞切除活性的转座元件。镶嵌回复体的色素沉着模式表明黑色素的频率与身体部位（例如眼睛、头部和背干）每单位面积的黑色素细胞数量一致。
The medaka fish albino mutant, i(1) is one of the Tomita collection of medaka pigmentation mutants which exhibits a complete albino phenotype, because of inactivation of the tyrosinase gene due to insertion of a transposable element, Tol-1. Recently, mosaic black-pigmented i(1) medaka fish have arisen in one of our laboratory breeding populations. Their pigmented cells have been observed in all of the tissues, including the eye and skin, in which melanin is detectable in the wild type. In this study, we analyzed the tyrosinase gene of revertants and showed Tol-1 to have been precisely excised from the gene, suggesting a causal relationship. Mosaic patterns of pigmentation indicate spontaneous somatic excision of the element from the tyrosinase gene. To our knowledge, this is the first transposable element with somatic excision activity demonstrated phenotypically in vertebrates. The pattern of pigmentation in mosaic revertants indicates frequencies of melanin pigments to be consistent with the numbers of melanophores per unit area of body sites, such as the eyes, head and dorsal trunk.