Preliminary Study of Orthogonal Electrochromatography for Simultaneous Two-Dimensional Separation Using a Monolithic Polymer Layer
Preliminary Study of Orthogonal Electrochromatography for Simultaneous Two-Dimensional Separation Using a Monolithic Polymer Layer
复制标题
使用整体聚合物层同时进行二维分离的正交电色谱的初步研究
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
H. Ohtani
中科院分区：
文献类型：
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作者：
Hiroaki Ito;Mika Matsugi;S. Kitagawa;H. Ohtani
本文研究了一种整体式聚合物膜作为固定相，并将其与两个电极相结合，用于高效液相色谱和正交电泳的同时二维分离。通过两步紫外光引发的光聚合反应在一对玻璃板之间制备整体聚合物层。在制备用于分离的整料聚合物层之前，沿着电极制备高密度整料棒以抑制在电极处形成的气泡渗透到分离整料中。利用整体聚合物膜，将梯度高效液相色谱和正交电泳相结合，成功实现了有色染料的二维分离。还展示了蛋白质的二维分离。
Fabrication of a monolithic polymer layer, as the stationary phase, equipped with two electrodes was studied for simultaneous two-dimensional separation, combining high performance liquid chromatography and orthogonal electrophoresis. The monolithic polymer layer was prepared between a pair of glass plates by a two-step UV-initiated photo-polymerization. Prior to preparation of the monolithic polymer layer for separation, high-density monolith bars were prepared along the electrodes to suppress the penetration of bubbles formed at the electrodes into the separation monolith. Using the monolithic polymer layer, 2D separation of colored-dyes was successfully achieved by combining gradient HPLC and orthogonal electrophoresis. The 2D separation of proteins was also demonstrated.
DOI：
10.1016/j.chroma.2011.07.019
发表时间：
2012-03-09
期刊：
Journal of chromatography. A
影响因子：
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作者：
Svec F
通讯作者：
Svec F
影响因子：
62.1
作者：
Zhang, Yaoyang;Fonslow, Bryan R.;Shan, Bing;Baek, Moon-Chang;Yates, John R., III
通讯作者：
Yates, John R., III