Preparation and characterization of bovine serum albumin surface-imprinted thermosensitive magnetic polymer microsphere and its application for protein recognition
Preparation and characterization of bovine serum albumin surface-imprinted thermosensitive magnetic polymer microsphere and its application for protein recognition
复制标题
牛血清白蛋白表面印迹热敏磁性聚合物微球的制备、表征及其在蛋白质识别中的应用
DOI：
10.1016/j.bios.2013.07.008
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发表时间：
2014-01-15
影响因子：
12.6
通讯作者：
Zhang, Qiuyu
中科院分区：
文献类型：
--
作者：
Li, Xiangjie;Zhang, Baoliang;Zhang, Qiuyu
以温敏性单体N-异丙基丙烯酰胺（NIPAM）、功能性单体甲基丙烯酸（MAA）和交联剂N，N '-亚甲基双丙烯酰胺（MBA）为原料，采用表面接枝共聚法制备了牛血清白蛋白表面印迹温敏磁性复合微球。采用透射电镜（TEM）、傅里叶变换红外光谱（FT-IR）、振动样品磁强计（VSM）和热重分析（TGA）等手段对热敏磁性分子印迹微球的结构和组成进行了表征。温敏性、吸附容量、选择性和重复使用性的研究结果表明，在Fe3O4@SiO2表面形成了温敏性接枝聚合物层P（NIPAM MAA MBA），对模板蛋白具有良好的吸附容量和特异性识别。当吸附温度高于聚N-异丙基丙烯酰胺（PNIPAM）的最低临界溶解温度（LCST）时，印迹孔的形状记忆效应更明显。也就是说，在此条件下更有利于模板分子的捕获，印迹因子也会更高。另一方面，当脱附温度低于PNIPAM的LCST时，印迹空腔与模板分子之间的形状记忆效应降低，有利于模板分子从印迹空腔中释放出来。基于这一性质，热敏印迹层的存在可以间接地调节模板分子的吸附和脱附。(C)2013爱思唯尔有限公司版权所有。
A novel bovine serum albumin surface-imprinted thermosensitive magnetic composite microsphere was successfully prepared by the surface grafting copolymerization method in the presence of temperature-sensitive monomer N-isopropylacrylamide (NIPAM), functional monomer methacrylic acid (MAA) and cross-linking agent N,N'-methylenebisacrylamide (MBA). The structure and component of the thermosensitive magnetic molecularly imprinted microsphere were investigated by transmission electron microscopy (TEM), Fourier transform infrared (FT-IR), vibrating sample magnetometer (VSM) and thermogravimetric analysis (TGA). The results of thermosensitivity, adsorption capacity, selectivity and reusability showed the formation of a thermosensitivity grafting polymer layer P(NIPAM MAA MBA) on the surface of Fe3O4@SiO2 and the good adsorption capacity and specific recognition for template protein. When the adsorption temperature was higher than the lower critical solution temperature (LCST) of poly (N-isopropylacrylamide) (PNIPAM), shape memory effect of imprinted cavities would be more effective. In other words, it was more conducive to capture template molecules under this condition and the imprinting factor would be higher. On the other hand, when the desorption temperature was lower than LCST of PNIPAM, the decrease of shape memory effect between imprinted cavities and template molecules would facilitate the release of template molecules from the imprinted cavities. Based on this property, the adsorption and desorption of template molecules could be regulated by system temperature indirectly which benefited from the existence of thermosensitivity imprinting layer. (C) 2013 Elsevier B.V. All rights reserved.