Smartphone-based portable electrochemical biosensing system for detection of circulating microRNA-21 in saliva as a proof-of-concept
Smartphone-based portable electrochemical biosensing system for detection of circulating microRNA-21 in saliva as a proof-of-concept
复制标题
基于智能手机的便携式电化学生物传感系统，用于检测唾液中循环的 microRNA-21，作为概念验证
DOI：
10.1016/j.snb.2020.127718
复制
发表时间：
2020-04-01
影响因子：
8.4
通讯作者：
Liu, Qingjun
中科院分区：
文献类型：
--
作者：
Low, Sze Shin;Pan, Yixin;Liu, Qingjun
循环microRNAs(MiRNAs)是有效的癌症生物标志物，在体液中高度稳定，能够进行非侵入性检测。便携式、低成本和高效的生物传感系统对于早期现场诊断至关重要，特别是在资源有限的情况下。目前的工作是开发一种基于智能手机的生物传感系统，用于电化学检测唾液中循环microRNA-21(miR-21)生物标志物作为概念验证。基于智能手机的生物传感系统由一次性丝网印刷生物传感器、检测电路板和配备特殊设计的Android应用程序的蓝牙智能手机组成。在rGO/Au修饰电极上，miR-21靶与单链DNA探针杂交，峰电流随miR-21靶浓度的增加而减小。基于智能手机的生物传感系统与商用电化学工作站的检测性能相当，在1×10(-4)M到1×10(-1)(2)M的浓度范围内检测miR-21，相关系数(r(2))为0.99。除了对错配和非靶序列有良好的选择性外，该生物传感系统在人工唾液中的回收率也在96.2%-107.2%之间。基于智能手机的生物传感系统有望实现快速、高灵敏地检测体液中循环的miRNA生物标记物，用于偏远地区的护理点检测。
Circulating microRNAs (miRNAs) are effective cancer biomarkers that are highly stable in body fluids enabling noninvasive detection. Portable, low-cost and efficient biosensing systems is crucial for early on-site diagnosis, especially in resource-limited settings. Present work deals with the development of a smartphone-based biosensing system for electrochemical detection of circulating microRNA-21 (miR-21) biomarker in saliva as a proof-of-concept. The smartphone-based biosensing system composed of a disposable screen-printed biosensor modified with reduced graphene oxide/gold (rGO/Au) composite, a circuit board for detection and a Bluetoothenabled smartphone equipped with specially designed Android application. The hybridization between miR-21 target and ssDNA probe on the rGO/Au-modified electrode resulted in the decrease in peak current with increase in miR-21 target concentration. The smartphone-based biosensing system showed comparable performance with commercial electrochemical workstation, for miR-21 detection in the concentration range of 1 x 10(-4) M to 1 x 10(-1)(2) M with correlation coefficient (r(2)) of 0.99. In addition to good selectivity in discriminating mismatched and non-target sequences, the biosensing system also showed acceptable recovery rate ranging from 96.2%-107.2 % in spiked artificial saliva. The smartphone-based biosensing system is envisioned to be able to realize rapid and highly sensitive detection of circulating miRNA biomarker in body fluid for point-of-care testing in remote areas.