Latitudinal and intracolony ITS‐rDNA sequence variation in the symbiotic dinoflagellate genus Symbiodinium (Dinophyceae) in Zoanthus sansibaricus (Anthozoa: Hexacorallia)
Latitudinal and intracolony ITS‐rDNA sequence variation in the symbiotic dinoflagellate genus Symbiodinium (Dinophyceae) in Zoanthus sansibaricus (Anthozoa: Hexacorallia)
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DOI：
10.1111/j.1440-1835.2006.00419.x
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发表时间：
2006-06
影响因子：
1.5
通讯作者：
J. Reimer;K. Takishita;S. Ono;T. Maruyama;J. Tsukahara
中科院分区：
文献类型：
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作者：
J. Reimer;K. Takishita;S. Ono;T. Maruyama;J. Tsukahara
我们测定了共生菌核糖体DNA内转录间隔区(ITS-rDNA)的序列。(Freudenthal)在日本沿纬度梯度的同种结缕草(Carlgren)群体。系统发育分析表明，在北边的Kokubu和Sakurajima，Zoantus仅含有共生线虫亚门C1，而Yakushima Zoantus则含有共生线虫C1和C15亚门，最南端的Amami Zoantus共生线虫则为A1和C1亚门，显示出全岛的灵活性。单独的结缕草群体只与一个亚枝相关，但出人意料的是，从单个结缕草群体中获得的共生菌ITS-rDNA克隆序列之间存在微小的差异。其中一个克隆在ITS-2/28S rDNA交界区有较大的缺失，在另一个克隆的5.8S rDNA有另一个大的缺失。我们克隆内序列的异质性可能是由于在单个共生菌基因组中存在多个拷贝的ITS-rDNA区域的结果，也可能是由于宿主中可能存在密切相关的共生菌基因型的结果。
We sequenced the internal transcribed spacer of ribosomal DNA (ITS‐rDNA) of Symbiodinium spp. (Freudenthal) from conspecific Zoanthus sansibaricus (Carlgren) colonies along a latitudinal gradient in Japan. Phylogenetic analysis reveals that Zoanthus in the two northern sites of Kokubu and Sakurajima harbor exclusively Symbiodinium subclade C1, whereas Yakushima Zoanthus harbors Symbiodinium subclades C1 and C15, and southernmost Amami Zoanthus Symbiodinium subclades A1 and C1, indicating holobiont flexibility. Individual Zoanthus colonies associated exclusively with one single subclade, but unexpectedly there was small variation between Symbiodinium ITS‐rDNA clone sequences obtained from within individual Zoanthus colonies. There was also a large deletion in the ITS‐2/28S rDNA boundary region in one clone sequence, and another large deletion in the 5.8S rDNA region in another clone. Our intracolony sequence heterogeneity might be a result of the presence of multiple copies of the ITS‐rDNA region within individual Symbiodinium genomes, or result from the possible presence of closely related Symbiodinium genotypes in the host.