CD4+ T cell epitope affinity to MHC II influences the magnitude of CTL responses elicited by DNA epitope vaccines.
CD4+ T cell epitope affinity to MHC II influences the magnitude of CTL responses elicited by DNA epitope vaccines.
复制标题
CD4 T 细胞表位与 MHC II 的亲和力影响 DNA 表位疫苗引发的 CTL 反应的程度。
DOI：
10.1016/j.vaccine.2004.10.039
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
McMillan,Minnie
中科院分区：
文献类型：
--
作者：
Vatakis,DimitriosN;Koh,YiTing;McMillan,Minnie
Immunization with naked plasmid DNA elicits strong cell-mediated immune responses. In the present study, we examine strategies to enhance epitope-specific cytotoxic T lymphocyte (CTL) responses using DNA constructs, expressing a minimal class I epitope of the gp120 of HIV-IIIB. Here, we evaluate the effect of CD4+T cell (TH) epitope affinity for the MHC II molecule on the immunogenicity of our DNA vaccines. Our data indicate that a low-affinity THepitope decreased the magnitude of the CTL responses. In addition, we observed decreased numbers of epitope-specific T helper cells and CTLs, as well as diminished cytokine secretion and proliferative responses. Thus, the immunogenicity of a DNA epitope vaccine can be modulated by altering the affinity of the THepitope.
