Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids
Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids
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DOI：
10.1002/rcm.2243
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发表时间：
2005-01-01
影响因子：
2
通讯作者：
Sunner, J
中科院分区：
文献类型：
--
作者：
Shiea, J;Huang, MZ;Sunner, J
电喷雾辅助激光解吸/电离（ELDI）质谱是一种将激光解吸与电喷雾后电离相结合的新方法，可用于固体物质的快速分析。使用脉冲氮激光器从固体金属和绝缘基质中解吸分析物。后电离产生高质量的质谱特征的电喷雾，包括蛋白质多重充电。第一次，完整的蛋白质的质谱使用激光解吸获得不添加基质。牛细胞色素c和一种含有安眠酮的违禁药物被选择在这项研究中，以证明ELDI的蛋白质和合成的有机化合物的分析的适用性。版权所有（c）2005年约翰威利父子有限公司。
A new method of electrospray-assisted laser desorption/ionization (ELDI) mass spectrometry, which combines laser desorption with post-ionization by electrospray, was applied to rapid analysis of solid materials under ambient conditions. Analytes were desorbed from solid metallic and insulating substrata using a pulsed nitrogen laser. Post-ionization produced high-quality mass spectra characteristic of electrospray, including protein multiple charging. For the first time, mass spectra of intact proteins were obtained using laser desorption without adding a matrix. Bovine cytochrome c and an illicit drug containing methaqualone were chosen in this study to demonstrate the applicability of ELDI to the analysis of proteins and synthetic organic compounds. Copyright (c) 2005 John Wiley & Sons, Ltd.