Post-transfusion hepatitis type B following multiple transfusions of HBsAg-negative blood
Post-transfusion hepatitis type B following multiple transfusions of HBsAg-negative blood
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DOI：
10.1016/s0168-8278(96)80232-7
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发表时间：
1996-11-01
影响因子：
25.7
通讯作者：
Naik, S
中科院分区：
文献类型：
--
作者：
Saraswat, S;Banerjee, K;Naik, S
背景/目的：即使在对供血者的血液进行 HBsAg、抗 HBc、抗 HCV 和丙氨酸氨基转移酶活性筛查后，输血后肝炎仍然持续发生，但频率有所下降。来自发展中国家的关于输血后肝炎发生频率和类型的数据很少。我们进行了这项前瞻性研究，旨在通过 ELISA 确定输血 HBsAg 阴性的血液后，我们中心的输血后肝炎的发生率和类型。方法：41 名患者接受了对接受 3 个或更多单位 HBsAg 阴性血液的心脏直视手术患者进行随访，将输给发生输血后乙型肝炎的受者的供体单位的血清样本通过聚合酶链式反应进行 HBV DNA 扩增，使用两组 X 基因特异性引物，扩增 HBV DNA 的 250 bp 片段。 结果：我们发现 41 名患者中有 6 名（14.6％）发生了输血后肝炎；其中 4 名（66.6%）出现了输血后乙型肝炎，而 2 名（33.3%）出现了输血后丙型肝炎。这 6 名受血者总共接受了 48 单位的血液，这 48 单位中的 30 单位可以通过聚合酶链式反应进行 HBV DNA 扩增。11 份供体样本呈聚合酶链式反应阳性，并已输给 4 名出现输血后乙型肝炎的患者中的 3 名。结论：我们得出的结论是，输血后乙型肝炎仍然是印度输血后肝炎的最常见原因，通过 ELISA 筛查供体单位的 HBsAg 并不能排除所有具有乙型肝炎病毒感染性的血液单位，应采取其他措施确保血液供应安全。
Background/Aims: Post-transfusion hepatitis continues to occur, though with decreasing frequency, even after screening donor blood for HBsAg, anti-HBc, anti-HCV and alanine aminotransferase activity, Data from developing countries on the frequency and type of post-transfusion hepatitis are scarce, We undertook this prospective study to determine the incidence and type of post-transfusion hepatitis at our center after transfusion of blood negative for HBsAg by ELISA.Methods: Forty-one patients undergoing open-heart surgery, who had received 3 or more units of HBsAg-negative blood, were followed up, Serum samples of donor units transfused to recipients who developed post-transfusion hepatitis-B were subjected to HBV DNA amplification by the polymerase chain reaction, using two sets of X-gene specific primers which amplified a 250-bp fragment of the HBV DNA.Results: We found that six of the 41 patients (14.6%) developed post-transfusion hepatitis; four of them (66.6%) developed icteric post-transfusion hepatitis B, whereas two (33.3%) developed anicteric post-transfusion hepatitis C. These six recipients received a total of 48 units of blood and 30 of these 48 units could be subjected to HBV DNA amplification by polymerase chain reaction, Eleven donor samples were polymerase chain reaction positive and had been transfused to three of the four patients who had developed post-transfusion hepatitis B.Conclusions: We conclude that post-transfusion hepatitis B continues to be the most common cause of post-transfusion hepatitis in India, Screening of donor units for HBsAg by ELISA does not exclude all blood units infectious for hepatitis B virus, Additional measures to ensure safety of blood supply should be sought.