Genetic structure of Amphioctopus fangsiao (Mollusca, Cephalopoda) in Chinese waters inferred from variation in three mtDNA genes (ATPase 6, ND2, and ND5)
Genetic structure of Amphioctopus fangsiao (Mollusca, Cephalopoda) in Chinese waters inferred from variation in three mtDNA genes (ATPase 6, ND2, and ND5)
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从三个 mtDNA 基因（ATPase 6、ND2 和 ND5）的变异推断中国水域中的方蛸（软体动物、头足类）的遗传结构
DOI：
10.1007/s10750-019-03981-9
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发表时间：
2019-07-01
期刊：
影响因子：
2.6
通讯作者：
Lu, Zhen-ming
中科院分区：
文献类型：
--
作者：
Muhammad, Faiz;Chen, Wei;Lu, Zhen-ming
长蛸（Amphioctopus fangsiao）是中国沿海水域的一种具有重要经济价值的资源。利用三个线粒体DNA基因评估了中国沿海从北到南九个种群的遗传结构。分别从183、170和167个个体中扩增出代表三个基因（ATP酶6、ND2和ND5）的长度为575、639和640bp的片段。总体而言，ATP酶6、ND2和ND5表现出较高的单倍型多样性和较低的核苷酸多样性（单倍型多样性HD：0.683 - 0.896；核苷酸多样性π：0.021 - 0.033）。所有三个线粒体DNA基因均显示种群间分子方差较高，种群内方差较低。ATP酶6（ΦST = 0.000至0.997）、ND2（ΦST = 0.000至0.997）和ND5（ΦST = 0.125至0.983）的ΦST值表明种群间存在分化。构建的系统发育树和单倍型网络将九个种群分为两个分支，分别代表北部种群和南部种群。长江河口的低盐度可能作为一种屏障促进了两个分支之间的分化。这些结果增进了我们对长蛸遗传结构的理解，并有助于其遗传资源的管理。
Amphioctopus fangsiao is an economically important resource found in Chinese coastal waters. Three mitochondrial DNA genes were used to assess the genetic structure among nine populations spanning the northern to southern regions of the Chinese coast. Fragments of 575, 639, and 640 bp in length representing three genes (ATPase 6, ND2, and ND5) were amplified from 183, 170, and 167 individuals, respectively. Overall, ATPase 6, ND2, and ND5 showed high haplotype diversity and low nucleotide diversity (HD: 0.683–0.896; −π: 0.021–0.033). All three mtDNA genes revealed high molecular variance among populations and low variance within populations. ΦST values obtained for ATPase 6 (ΦST = 0.000 to 0.997), ND2 (ΦST = 0.000 to 0.997), and ND5 (ΦST = 0.125 to 0.983) showed differentiation among the populations. The constructed phylogeographic tree and haplotype network separated the nine populations into two clades representing the northern and southern populations. Low salinity in the Changjiang River estuary may act as a barrier to promote the differentiation between the two clades. These results enhance our understanding of the genetic structure of A. fangsiao and can promote the management of its genetic resources.