Characterization of RNA Nanoparticles and Their Dynamic Properties Using Atomic Force Microscopy.
Characterization of RNA Nanoparticles and Their Dynamic Properties Using Atomic Force Microscopy.
复制标题
使用原子力显微镜表征 RNA 纳米颗粒及其动态特性。
DOI：
10.1007/978-1-0716-3417-2_12
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Krasnoslobodtsev,AlexeyV
中科院分区：
文献类型：
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作者：
Lushnikov,AlexanderJ;Avila,YelixzaI;Afonin,KirillA;Krasnoslobodtsev,AlexeyV
本章中描述的协议允许获取基于RNA的纳米环结构的形貌图像，并使用原子力显微镜（AFM）成像评估其动态特性。原子力显微镜是一个不可或缺的工具，用于表征核酸为基础的纳米结构与观察复合物在几个纳米范围内的特殊能力。这种方法可以可视化结构特征，并评估单个结构不同的RNA纳米环之间的差异。由于高分辨率的AFM形貌图像，我们介绍了一种方法，允许区分不服从其他实验技术的RNA纳米粒子的动态行为的差异。该方案详细描述了RNA纳米结构的制备过程，AFM成像和数据分析。
The protocol described in this chapter allows for acquiring topography images of RNA-based nanoring structures and assessing their dynamic properties using atomic force microscopy (AFM) imaging. AFM is an indispensable tool for characterization of nucleic acid-based nanostructures with the exceptional capability of observing complexes in the range of a few nanometers. This method can visualize structural characteristics and evaluate differences between individual structurally different RNA nanorings. Due to the highly resolved AFM topography images, we introduce an approach that allows to distinguish the differences in the dynamic behavior of RNA nanoparticles not amenable to other experimental techniques. This protocol describes in detail the preparation procedures of RNA nanostructures, AFM imaging, and data analysis.