Nucleotide sequence of a cDNA for mouse cytochrome c oxidase subunit VIIc.
Nucleotide sequence of a cDNA for mouse cytochrome c oxidase subunit VIIc.
复制标题
小鼠细胞色素c氧化酶亚基VIIc的cDNA的核苷酸序列。
作者：
M. Akamatsu;L. Grossman
细胞色素c氧化酶（考克斯），电子传递链的复合物IV，在两个细胞隔室中编码。哺乳动物酶的13个亚基由线粒体DNA编码的3个亚基和核DNA编码的10个亚基组成。核编码亚基的功能尚不清楚，但已被认为可调节复合物的活性（综述见1）。我们一直在分离小鼠细胞核编码亚基的cDNA用于后续研究。我们在λ ZAP中筛选了小鼠（BALB/c）心脏cDNA文库（Stratagene），该文库含有先前分离的牛VIIc亚基cDNA（2）。我们分离的cDNA的插入片段为391 bp（不包括接头，如下括号中所示）。推导的16个氨基酸的前序列与牛（2）和人（3）相似;成熟多肽（起始于“）与牛考克斯VIIc（不同的氨基酸加下划线一次）有94%的同一性，与人亚基（差异加双下划线）有81%的同一性。两个物种的差异用=表示。一个假定的多聚腺苷酸化信号被加下划线。
Cytochrome c oxidase (COX), complex IV of the electron transport chain, is encoded in two cellular compartments. The thirteen subunits of the mammalian enzyme consist of three coded by mitochondrial DNA and ten by nuclear DNA. The function(s) of the nuclear encoded subunits is unknown, but has been suggested to modulate the activity of the complex (reviewed in 1). We have been isolating mouse cDNAs for nuclear encoded subunits for subsequent studies. We screened a mouse (BALB/c) heart cDNA library (Stratagene) in lambda ZAP with a previously isolated bovine cDNA for subunit VIIc (2). The insert of the cDNA we isolated was 391 bp (excluding linkers, shown below in brackets). The deduced presequence of 16 amino acids is like the bovine (2) and human (3); the mature polypeptide (starting at ") is 94% identical to bovine COX VIIc (amino acids that differ are underlined once) and 81% identical to the human subunit (double underline for differences). A difference in both species is indicated by =. A presumptive polyadenylation signal is overlined.