In vitro binding of natural killer cells to Cryptococcus neoformans targets.
In vitro binding of natural killer cells to Cryptococcus neoformans targets.
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自然杀伤细胞与新型隐球菌靶标的体外结合。
DOI：
10.1128/iai.50.1.50-57.1985
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发表时间：
1985
影响因子：
3.1
通讯作者：
Murphy,JW
中科院分区：
文献类型：
--
作者：
Nabavi,N;Murphy,JW
在不连续Percoll梯度上进一步分离来自7至8周龄CBA小鼠的尼龙毛非粘附脾细胞。通过形态学检查、免疫荧光染色和在4-h51 Cr释放试验中评估各Percoll细胞组分对YAC-1靶标的细胞溶解活性，证实了Percoll组分1和2中自然杀伤（NK）细胞的富集。通过使用体外18小时生长抑制试验，检测从每个Percoll组分中分离的细胞对新型隐球菌（一种致病性酵母样生物）的生长抑制活性。结果表明，在Percoll级分1和2中，NK细胞富集伴随着抗隐球菌活性的富集。来自NK细胞富集级分的细胞与肌球蛋白靶点形成缀合物，类似于NK细胞-肿瘤系统中报道的缀合物。此外，效应细胞-隐球菌结合物的百分比与C.所用效应细胞的新生动物生长抑制活性。效应细胞-隐球菌结合物的扫描电子显微镜显示效应细胞和隐球菌靶之间的直接接触。免疫标记结合扫描电镜观察表明，该效应细胞与C。新生儿为去唾液酸GM 1阳性，因此具有NK细胞特征。
Nylon wool-nonadherent splenic cells from 7- to 8-week-old CBA mice were further fractionated on discontinuous Percoll gradients. Enrichment of natural killer (NK) cells in Percoll fractions 1 and 2 was confirmed by morphological examination, by immunofluorescent staining, and by assessing the cytolytic activity of each Percoll cell fraction against YAC-1 targets in the 4-h51Cr release assay. Cells isolated from each Percoll fraction were tested for growth-inhibitory activity against Cryptococcus neoformans, a pathogenic yeastlike organism, by using an in vitro 18-h growth inhibition assay. The results showed that NK cell enrichment was concomitant with enrichment of anti-Cryptococcus activity in Percoll fractions 1 and 2. Cells from NK cell-rich fractions formed conjugates with the mycotic targets similar to the conjugates reported in NK cell-tumor systems. In addition, the percentage of effector cell-Cryptococcus conjugates was directly proportional to the level of the C. neoformans growth-inhibitory activity of the effector cells used. Scanning electron microscopy of the effector cell-Cryptococcus conjugates showed direct contact between the effector cells and the cryptococcal targets. An immunolabeling method combined with scanning electron microscopy was used to demonstrate that the effector cells attached to C. neoformans were asialo GM1 positive and, therefore, had NK cell characteristics.
自然杀伤 (NK) 细胞对新型隐球菌的体外反应性。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Murphy,JW;McDaniel,DO
通讯作者：
McDaniel,DO
影响因子：
6.4
作者：
LEVITZ, SM;DIAMOND, RD
通讯作者：
DIAMOND, RD