SpectraBank: An open access tool for rapid microbial identification by MALDI-TOF MS fingerprinting
SpectraBank: An open access tool for rapid microbial identification by MALDI-TOF MS fingerprinting
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DOI：
10.1002/elps.201200074
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发表时间：
2012-07-01
期刊：
影响因子：
2.9
通讯作者：
Calo-Mata, Pilar
中科院分区：
文献类型：
--
作者：
Boehme, Karola;Fernandez-No, Inmaculada C.;Calo-Mata, Pilar
MALDI-TOF MS已被证明是一种准确、快速和具有成本效益的微生物鉴定技术，其中未知菌株的光谱指纹可以与参考菌株的光谱数据库进行比较。大多数现有的数据库都是私人的，访问起来往往很昂贵，研究人员之间共享的光谱信息很少。本通讯的目的是介绍SpectraBank数据库（），该数据库提供来自各种微生物的开放存取MALDI-TOF质谱。这项工作旨在让读者熟悉SpectraBank数据库，从样品制备，数据收集和数据分析到光谱参考数据如何用于微生物物种鉴定。该数据库目前包括来自70多个细菌物种的200多个MALDI-TOF MS光谱，并链接到免费提供的基于网络的应用程序SPECLUST（），以比较获得的峰质量列表并评估细菌蛋白质组学关系。SpectraBank数据库旨在通过添加我们实验室和其他研究人员获得的微生物菌株的新光谱来扩展。
MALDI-TOF MS has proved to be an accurate, rapid, and cost-effective technique for microbial identification in which the spectral fingerprint of an unknown strain can be compared to a database of spectra from reference strains. Most of the existing databases are private and often costly to access, and little spectral information is shared among researchers. The objective of the present communication is to introduce the SpectraBank database (), which provides open access MALDI-TOF mass spectra from a variety of microorganisms. This work aims to familiarize readers with the SpectraBank database, from the sample preparation, data collection, and data analysis to how the spectral reference data can be used for microbial species identification. The database currently includes more than 200 MALDI-TOF MS spectra from more than 70 bacterial species and links to the freely available web-based application SPECLUST () to allow comparisons of the obtained peak mass lists and evaluate phyloproteomic relationships. The SpectraBank database is intended to be expanded by the addition of new spectra from microbial strains, obtained in our laboratory and by other researchers.