Detection of potato mop-top virus capsid readthrough protein in virus particles
Detection of potato mop-top virus capsid readthrough protein in virus particles
复制标题
DOI：
10.1099/0022-1317-78-7-1779
复制
发表时间：
1997-07-01
影响因子：
3.8
通讯作者：
Reavy, B
中科院分区：
文献类型：
--
作者：
Cowan, GH;Torrance, L;Reavy, B
马铃薯口蹄疫病毒(PMTV)RNA3编码20 kDa的外壳蛋白和67 kDa的较大通读蛋白。通读蛋白通过抑制琥珀终止密码子来表达，琥珀终止密码子终止了外壳蛋白基因。克隆了全长为21 kDa的通读蛋白C端片段，与谷胱甘肽S转移酶融合，在大肠杆菌中表达。用纯化的融合蛋白制备的抗血清用于检测PMTV感染叶片提取液中的通读蛋白。免疫金标记法研究表明，通读蛋白位于某些病毒颗粒的一端附近。
Potato mop-top furovirus (PMTV) RNA 3 encodes the 20 kDa coat protein and a larger readthrough protein of 67 kDa. The readthrough protein is expressed by suppression of the amber stop codon which terminates the coat protein gene. A 21 kDa C-terminal fragment of the readthrough protein was cloned, fused to glutathione S-transferase and expressed in E. coli. An antiserum prepared against purified fusion protein was used in ELISA to detect the readthrough protein in extracts of PMTV-infected leaves. Immunogold labelling studies showed that the readthrough protein was located near one extremity of some of the virus particles.