Expression profiles of peroxiredoxins in liver stage of the rodent malari a parasite Plasmodium berghei
Expression profiles of peroxiredoxins in liver stage of the rodent malari a parasite Plasmodium berghei
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啮齿动物疟原虫伯氏疟原虫肝脏阶段过氧化还原蛋白的表达谱
DOI：
10.1016/j.parint.2012.11.007
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发表时间：
2013
影响因子：
1.9
通讯作者：
Kawazu S.
中科院分区：
文献类型：
--
作者：
Usui M;Masuda-Suganuma H;Fukumoto S;Angeles JM;Inoue N;Kawazu S.
采用定量逆转录- pcr （RT-PCR）和间接免疫荧光显微法（IFA）检测肝期伯氏疟原虫3种过氧化物还毒素（TPx-1、TPx-2和1-Cys Prx） mRNA和蛋白表达谱。RT-PCR实验显示，TPx-1 mRNA在孢子体感染后不久即表达，并保持表达至裂殖期。相比之下，1-Cys Prx的mRNA表达在寄生虫进入分裂体阶段时开始增加。用IFA法检测胞浆中的TPx-1和1-Cys Prx。这一发现提示了肝期寄生虫中胞质酶的发育阶段特异性表达。另一方面，TPx-2 mRNA的表达在滋养体时期开始增加，在分裂体时期达到峰值。在IFA中，发现TPx-2定位于线粒体。TPx-2的增加可能是由于寄生虫在分裂体阶段的指数发育需要ATP的产生，这可能会诱导线粒体中的活性氧（ROS）。
mRNA and protein expression profiles for three peroxiredoxins (TPx-1, TPx-2 and 1-Cys Prx) of liver stage Plasmodium berghei were examined through quantitative reverse transcription-PCR (RT-PCR) and indirect immunofluorescence microscopy assay (IFA). RT-PCR experiments revealed that mRNA expression for the TPx-1 was detected shortly after the sporozoite infection and kept expressed until the schizont stage. In contrast, the mRNA expression for 1-Cys Prx had begun increasing when the parasite developed into the schizont stage. Using the IFA, TPx-1 and 1-Cys Prx were detected in the cytosol. This finding suggested the developmental stage-specific expression of the cytosolic enzymes in the liver stage parasite. On the other hand, the mRNA expression for TPx-2 had begun increasing at the trophozoite stage and peaked at the schizont stage. In the IFA, TPx-2 was found localized in the mitochondria. The increase of TPx-2 might be explained by the exponential development of the parasite during the schizont stage requiring ATP production which may induce reactive oxygen species (ROS) in the mitochondria.