Adsorption and hydrolytic activity of lysozyme on diamond nanocrystallites
Adsorption and hydrolytic activity of lysozyme on diamond nanocrystallites
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DOI：
10.1016/j.diamond.2007.01.030
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发表时间：
2007-04-01
影响因子：
4.1
通讯作者：
Wu, Victor Wei-Keh
中科院分区：
文献类型：
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作者：
Nguyen, T.-Thanh-Bao;Chang, Huan-Cheng;Wu, Victor Wei-Keh
氧化酸处理的纳米金刚石对蛋白质具有高亲和力，这是一种非常适合用于生物技术应用的酶固定化的特性。使用溶菌酶作为一个例子，这项工作表明，酶可以保留其大部分的活性后，物理吸附到100纳米金刚石微晶的表面。在最大表面覆盖率为50%和pH为5时，相对于溶液中游离溶菌酶的活性接近60%。虽然酶活性随着表面覆盖度的降低而降低，但可以通过用补充蛋白质如细胞色素c封闭表面上的空位点来恢复酶活性，以创造更“拥挤”的环境。在20%的部分覆盖度下，可以获得高达70%的相对活性。(C)2007 Elsevier B.V.保留所有权利。
Oxidative-acid-treated nanodiamonds exhibit high affinity for proteins, a property well suited for immobilization of enzymes for biotechnological application. Using lysozyme as an example, this work demonstrates that the enzyme can retain much of its activity after physical adsorption to the surfaces of 100-nm diamond crystallites. The activity relative to that of free lysozyme in solution is similar to 60% at the maximum surface coverage of 50% and pH 5. While the enzymatic activity decreases as the surface coverage is lowered, it can be recovered by blocking the empty sites on the surface with supplementary proteins such as cytochrome c to create a more "crowded" environment. A relative activity up to 70% can be attained at a partial coverage of 20%. (C) 2007 Elsevier B.V. All rights reserved.