Changes in related circular RNAs following ERβ knockdown and the relationship to rBMSC osteogenesis
Changes in related circular RNAs following ERβ knockdown and the relationship to rBMSC osteogenesis
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ERβ 敲低后相关环状 RNA 的变化及其与 rBMSC 成骨的关系
DOI：
10.1016/j.bbrc.2017.09.068
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发表时间：
2017-11-04
影响因子：
3.1
通讯作者：
Yang, Li
中科院分区：
文献类型：
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作者：
Li, Xiaoyun;Peng, Bojia;Yang, Li
近年来，多项研究表明，环状RNA在多种疾病中发挥重要作用，但对雌激素受体β缺乏和环状RNA表达改变的时间顺序及其与成骨的关系知之甚少。在这里，我们通过蛋白质印迹和实时定量聚合酶链式反应分析表明，当ERβ被沉默时，成骨相关蛋白和mRNAs的表达下调。然后，我们进行RNA-Seq分析，以分析对照组和ERβ基因敲除组中CircRNA的差异表达。这一分析表明，146个CircRNA的差异表达符合折叠式变化=2.0，p
Recently, several studies have indicated that circular RNAs (circRNAs) play significant roles in various disease; however, little is known about the chronology of estrogen receptor beta (ER beta) deficiency and altered circRNA expression, or their relationship with osteogenesis. Herein, we show through western blot and quantitative real-time PCR assays, that when ER beta is silenced, the expression of osteogenesis-related proteins and mRNAs were down-regulated. We then performed RNA-Seq to analyze differential circRNA expression between the control and ER beta knockdown group. This analysis revealed that, 146 circRNAs were differentially expressed by fold-change >= 2.0, p